Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)
| Autor: | Gislayne de Paula Bueno, Peterson Trevisan Leivas, Camilla Borges Gazolla, Michelle Micarelli Struett, Kaleb Pretto Gatto, Mauricio Osvaldo Moura, Daniel Pacheco Bruschi |
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| Přispěvatelé: | Universidade Federal do Paraná (UFPR), Universidade Estadual Paulista (Unesp), Universidade Positivo (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Artificial Gene Amplification and Extension 01 natural sciences Genome Polymerase Chain Reaction Geographical locations Phylogeny Multidisciplinary biology Chromosome Biology Autosomes Chromosome Mapping Karyotype Genomics Microsatellite Medicine Anura Karyotypes Brazil Research Article Satellite DNA Science DNA Satellite Research and Analysis Methods 010603 evolutionary biology Chromosomes Evolution Molecular 03 medical and health sciences Cytogenetics Genetics Animals Repeated sequence Molecular Biology Techniques Molecular Biology Amphibian Genomics Gene Mapping Chromosome Biology and Life Sciences Cell Biology South America biology.organism_classification Chromosome Pairs 030104 developmental biology Cycloramphus Evolutionary biology Animal Genomics Cycloramphus bolitoglossus People and places Cloning |
| Zdroj: | PLoS ONE, Vol 16, Iss 1, p e0245128 (2021) PLoS ONE Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1932-6203 |
| Popis: | Made available in DSpace on 2021-06-25T10:50:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Cycloramphus bolitoglossus (Werner, 1897) is a rare species with a low population density in the Serra do Mar region of Paraná and Santa Catarina, in southern Brazil. Currently, it has been assigned to the Near Threatened (NT) category in the Brazilian List of Endangered Animal Species. Here, we described the karyotype of this species for the first time and investigated the patterns of some repetitive DNA classes in the chromosomes using molecular cytogenetic approaches. We isolated, sequenced and mapped the 5S rDNA and the satellite DNA PcP190 of C. bolitoglossus, as well as mapped the telomeric sequences and seven microsatellites motifies [(GA)15, (CA)15, (GACA)4, (GATA)8, (CAG)10, (CGC)10, and (GAA)]10. Cycloramphus bolitoglossus has 2n = 26 chromosomes and a fundamental number (FN) equal to 52, with a highly conserved karyotype compared to other genus members. Comparative cytogenetic under the phylogenetic context of genus allowed evolutionary interpretations of the morphological changes in the homologs of pairs 1, 3, and 6 along with the evolutionary history of Cycloramphus. Two subtypes of 5S rDNA type II were isolated in C. bolitoglossus genome, and several comparative analysis suggests mixed effects of concerted and birth-and-death evolution acting in this repetitive DNA. The 5S rDNA II subtype “a” and “b” was mapped on chromosome 1. However, their different position along chromosome 1 provide an excellent chromosome marker for future studies. PcP190 satellite DNA, already reported for species of the families Hylidae, Hylodidae, Leptodactylidae, and Odontophrynidae, is scattered throughout the C. bolitoglossus genome, and even non-heterochromatic regions showed hybridization signals using the PcP190 probe. Molecular analysis suggests that PcP190 satellite DNA exhibit a high-level of homogenization of this sequence in the genome of C. bolitoglossus. The PcP190 satDNA from C. bolitoglossus represents a novel sequence group, compared to other anurans, based on its hypervariable region. Overall, the present data on repetitive DNA sequences showed pseudogenization evidence and corroborated the hypothesis of the emergence of satDNA from rDNA 5S clusters. These two arguments that reinforced the importance of the birth-and-death evolutionary model to explain 5S rDNA patterns found in anuran genomes. Departamento de Genética Setor de Ciências Biológicas Universidade Federal do Paraná (UFPR) Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP) Curso de Ciências Biológicas Universidade Positivo (UP) Departamento de Zoologia Setor de Ciências Biológicas Universidade Federal do Paraná (UFPR) Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista (UNESP) |
| Databáze: | OpenAIRE |
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