Alcohol Metabolism Increases the Replication of Hepatitis C Virus and Attenuates the Antiviral Action of Interferon
| Autor: | Michael R. Beard, Ljiljana Semendric, Erin M. McCartney, Susan J Hinze, Steven A. Weinman, Brett Jones, Karla J. Helbig |
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| Rok vydání: | 2008 |
| Předmět: |
Hepatitis C virus
Hepacivirus Virus Replication medicine.disease_cause Gene Expression Regulation Enzymologic Virus Cell Line Interferon medicine Humans Immunology and Allergy Ethanol metabolism Ethanol biology virus diseases Cytochrome P-450 CYP2E1 CYP2E1 biology.organism_classification Virology digestive system diseases Oxidative Stress Infectious Diseases Viral replication Oxidative stress medicine.drug |
| Zdroj: | The Journal of Infectious Diseases. 198:1766-1775 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/593216 |
| Popis: | The interactions between hepatitis C virus (HCV) and alcohol metabolism are not well understood. To determine the effect that alcohol metabolism has on HCV replication and the antiviral action of interferon (IFN), Huh-7 cells that harbor HCV replication and metabolize ethanol via the introduced expression of cytochrome P450 2E1 (Cyp2e1) were treated with ethanol and IFN-alpha. Treatment of these cells with ethanol (0-100 mmol/L) significantly increased HCV replication. This effect was dependent on Cyp2e1 expression and alcohol-metabolized oxidative stress (OS), because the antioxidant N-acetylcysteine blocked this effect. Furthermore, the anti-HCV action of IFN-alpha was attenuated in the presence of ethanol metabolism, most likely via attenuation of Stat1 tyrosine-701 phosphorylation. These in vitro results mimic what is often noted clinically, and further dissection of this model system will aid in our understanding of interactions between HCV and alcohol metabolism. |
| Databáze: | OpenAIRE |
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