A recessive gene pepy-1 encoding Pelota confers resistance to begomovirus isolates of PepYLCIV and PepYLCAV in Capsicum annuum
| Autor: | Namiko Mori, Nadya Syafira Pohan, Elly Kesumawati, Atsushi J. Nagano, Mika Onouchi, Sota Koeda |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Locus (genetics) Genes Recessive Biology 01 natural sciences Chromosomes Plant Gene Expression Regulation Plant Pepper Genetics Tomato yellow leaf curl virus Gene Phylogeny Disease Resistance Plant Diseases Plant Proteins Begomovirus fungi Intron Chromosome Mapping food and beverages General Medicine biology.organism_classification Endonucleases genomic DNA Leaf curl Capsicum Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| DOI: | 10.21203/rs.3.rs-287025/v1 |
| Popis: | Pepper yellow leaf curl disease caused by begomoviruses seriously affects pepper (Capsicum spp.) production in a number of regions around the world. Ty genes of tomato, which confer resistance to the tomato yellow leaf curl virus, are the only begomovirus resistance genes cloned to date. In this study, we focused on the identification of begomovirus resistance genes in Capsicum annuum. BaPep-5 was identified as a novel source of resistance against pepper yellow leaf curl Indonesia virus (PepYLCIV) and pepper yellow leaf curl Aceh virus (PepYLCAV). A single recessive locus, which we named as pepper yellow leaf curl disease virus resistance 1 (pepy-1), responsible for PepYLCAV resistance in BaPep-5 was identified on chromosome 5 in an F2 population derived from a cross between BaPep-5 and the begomovirus susceptible accession BaPep-4. In the target region spanning 34 kb, a single candidate gene, the messenger RNA surveillance factor Pelota, was identified. Whole-genome resequencing of BaPep-4 and BaPep-5 and comparison of their genomic DNA sequences revealed a single nucleotide polymorphism (A to G) located at the splice site of the 9th intron of CaPelota in BaPep-5, which caused the insertion of the 9th intron into the transcript, resulting in the addition of 28 amino acids to CaPelota protein without causing a frameshift. Virus-induced gene silencing of CaPelota in the begomovirus susceptible pepper No.218 resulted in the gain of resistance against PepYLCIV, a phenotype consistent with BaPep-5. The DNA marker developed in this study will greatly facilitate marker-assisted breeding of begomovirus resistance in peppers. |
| Databáze: | OpenAIRE |
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