Cholesterol movement between human skin fibroblasts and phosphatidylcholine vesicles
| Autor: | Sandra Czekanski, Mark J. Poznansky |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Biophysics Mevalonic Acid Biology Biochemistry chemistry.chemical_compound Phosphatidylcholine medicine Humans Carbon Radioisotopes Fibroblast Cells Cultured Skin Liposome Cholesterol Vesicle Reverse cholesterol transport Infant Newborn Cell Biology Fibroblasts Kinetics medicine.anatomical_structure Membrane chemistry Liposomes Phosphatidylcholines lipids (amino acids peptides and proteins) Intracellular |
| Zdroj: | Biochimica et biophysica acta. 685(2) |
| ISSN: | 0006-3002 |
| Popis: | Cholesterol readily exchanges between human skin fibroblasts and unilamellar phospholipid vesicles. Only a fraction of the exchangeable cholesterol and only 10-15% of the total cellular free cholesterol is available for net movement or depletion to cholesterol-free phosphatidylcholine vesicles. [14C]Cholesterol introduced into the fibroblast plasma membrane by exchange from lipid vesicles does not readily equilibrate with fibroblast cholesterol labelled endogenously from [3H]mevalonic acid. While endogenously-synthesized [3H]cholesterol readily becomes incorporated into a pool of esterified cholesterol, little, if any, of the [14C]cholesterol introduced into the fibroblast membrane by exchange from lipid vesicles becomes available for esterification. We interpret these findings as suggesting that: (1) net cholesterol movement from fibroblasts to an acceptor membrane is limited to a small percentage of the plasma membrane cholesterol, and (2) separate pools of cholesterol exist in human skin fibroblasts, one associated with the plasma membrane and the second associated with intracellular membranes, and equilibration of cholesterol between the two pools is a very limited process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|