Glucagon- and NPY-related peptide-immunoreactive cells in the gut of sea bass (Dicentrarchus labrax L.): a light and electron microscopic study
| Autor: | B. Agulleiro, Inés Gómez-Visus, Marı́a Teresa Lozano, Maria Pilar García-Hernández |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system
medicine.medical_specialty Glucagon-Like Peptides Fluorescent Antibody Technique Enteroendocrine cell Peptide Biology Glucagon Immunoenzyme Techniques Endocrinology Internal medicine mental disorders medicine Pancreatic polypeptide Animals Neuropeptide Y Sea bass Tyrosine Protein Precursors Microscopy Immunoelectron chemistry.chemical_classification digestive oral and skin physiology Glicentin Stomach Immunogold labelling Neuropeptide Y receptor humanities Peptide Fragments Intestines Microscopy Electron chemistry Animal Science and Zoology Bass hormones hormone substitutes and hormone antagonists |
| Zdroj: | General and comparative endocrinology. 112(1) |
| ISSN: | 0016-6480 |
| Popis: | Glucagon and peptide of the neuropeptide Y (NPY) family immunoreactivities were studied in the gut of sea bass (Dicentrarchus labrax) using antisera against bovine/porcine glucagon, porcine glucagon, glicentin (10–30), bovine pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), salmon PYY (sPYY), and NPY. Glucagon-, glicentin-, PYY-, and NPY-immunoreactive (ir) cells were detected in the stomach, and glucagon-, PP-, PYY-, sPYY-, and NPY-ir cells in the intestine. PP, PYY, and NPY immunoreactivities coexisted in intestinal endocrine cells (NPY-like peptide containing cells), in some of which there was also glucagon immunoreactivity. Preabsorption tests indicated that different products of the glucagon gene(s) are probably expressed in the stomach and intestine of sea bass and that the peptides belonging to the NPY family in the endocrine cells of the intestine are more similar to NPY than to other peptides of this family. Glucagon-ir cells in the stomach, and glucagon/NPY-like containing cells in the intestine, were characterized by conventional and immunogold electron-microscopic techniques. The glucagon cells had secretory granules with a clotted content, the gold particles being observed in both the core and the halo. Glucagon/NPY-like cells showed two types of secretory granules differing in size, both of which were immunogold labeled with anti-NPY and anti-sPYY; the smaller granules were weakly immunogold labeled with anti-glucagon. |
| Databáze: | OpenAIRE |
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