Alternative splicing of HLA class I transcripts induced by IFN-gamma and TNF in fibroblasts: release of soluble HLA class I heavy chain and an associate protein
| Autor: | Junming Le, Xiao He |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Immunology
Molecular Sequence Data Human leukocyte antigen Biology Polymerase Chain Reaction Exon Interferon-gamma HLA Antigens Humans Northern blot Cells Cultured Messenger RNA Base Sequence Tumor Necrosis Factor-alpha Alternative splicing Histocompatibility Antigens Class I RNA Fibroblasts Blotting Northern Molecular biology Precipitin Tests Transmembrane protein Blot Alternative Splicing Blotting Southern Autoradiography Electrophoresis Polyacrylamide Gel |
| Zdroj: | Cellular immunology. 162(1) |
| ISSN: | 0008-8749 |
| Popis: | FS-4 fibroblasts were found to produce 37-kDa HLA class I heavy chain in response to IFN-gamma or TNF in a time- and dose-dependent fashion, and a synergism between IFN-gamma and TNF was observed. Immunoprecipitation of IFN-gamma- or TNF-induced FS-4 cell culture supernatants by mAb A1.4 revealed an additional 33-kDa protein in association with the 37-kDa heavy chain. The 33-kDa protein appeared to be expressed in a 38-kDa form on the membrane of FS-4 cells induced by IFN-gamma or TNF, as A1.4 immunoprecipitated the 38-kDa band in association with the 44-kDa transmembrane HLA class I heavy chain. Release of the 37-kDa heavy chain could well be due to an alternative RNA splicing with the deletion of exon 5 encoding the hydrophobic transmembrane region of membrane-anchored HLA class I heavy chain. Northern blot analysis and S1 nuclease protection assay suggested the existence of HLA class I heavy chain mRNA lacking exon 5 in IFN-gamma- or TNF-induced FS-4 cells. Southern blot analysis on the products of reverse transcription-polymerase chain reaction amplification from cytoplasmic RNA confirmed induction of alternative splicing by these cytokines. Our results suggest that cytokine-induced production of soluble HLA class I molecules may play important roles in the regulation of T cell interaction with antigen-presenting cells. |
| Databáze: | OpenAIRE |
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