Endophytic fungi associated with Taxus fuana (West Himalayan Yew) of Pakistan: potential bio-resources for cancer chemopreventive agents
| Autor: | Safia Ahmed, Leng Chee Chang, Nighat Fatima, Hira Mehboob Mirza, Muniba Jadoon, Muneer Ahmed Qazi, John M. Pezzuto, Eun-Jung Park, Laura Marler, Ibrar Khan, Naima Atiq, Tamara P. Kondratyuk |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pharmaceutical Science Nitric Oxide Synthase Type II 01 natural sciences Plant use of endophytic fungi in defense 03 medical and health sciences Drug Discovery Botany Endophytes NAD(P)H Dehydrogenase (Quinone) Anticarcinogenic Agents Humans Pharmacology biology 010405 organic chemistry Aromatase Inhibitors NF-kappa B General Medicine Chaetomium biology.organism_classification Taxus fuana 0104 chemical sciences 030104 developmental biology Complementary and alternative medicine Taxus Trichoderma Penicillium MCF-7 Cells Molecular Medicine Potato dextrose agar Taxaceae |
| Zdroj: | Pharmaceutical biology. 54(11) |
| ISSN: | 1744-5116 |
| Popis: | Endophytic fungi, being a prolific source of bioactive secondary metabolites, are of great interest for natural product discovery.Isolation and partial characterization of endophytic fungi inhabiting the leaves and woody parts of Taxus fuana Nan LiR.R. Mill. (Taxaceae) and evaluation of biological activity.Endophytic fungal isolates were identified by molecular analysis of internal transcribed spacer (ITS) regions of 18S rDNA. Extracts of the endophytic fungi cultured on potato dextrose agar and modified medium were evaluated using cancer chemoprevention bioassays [inhibition of TNF-α-induced NFκB, aromatase and inducible nitric oxide synthase (iNOS); induction of quinone reductase 1 (QR1)] and growth inhibition with MCF-7 cells.Nine of 15 fungal isolates were identified as belonging to Epicoccum, Mucor, Penicillium, Chaetomium, Paraconiothriym, Plectania or Trichoderma. Five of the 15 extracts inhibited NFκB activity (ICThe endophytic fungi studied are capable of producing pharmacologically active natural compounds. In particular, isolates derived from the wood of Taxus fuana should be prioritized for the isolation and characterization of bioactive constituents. |
| Databáze: | OpenAIRE |
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