Neuroprotective Effects of A Standardized Flavonoid Extract of Safflower Against Neurotoxin-Induced Cellular and Animal Models of Parkinson's Disease
| Autor: | Wan Li, Xiao-Ping Pu, Yongfei Guo, Xin Zhao, Yingcong Ma, Chunyan Shi, Yi Sun, Nuramatjan Ablat, C. Wang, Hongbin Han, Hui Lei, Hanjie Jiang, Jiamin Xu, Xian-Rong Qi, Cao Jing, Min Ye, Rutong Ren |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Parkinson's disease Cell Survival Neurotoxins Carthamus tinctorius Substantia nigra Biology Pharmacology Neuroprotection Microtubules PC12 Cells Article Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Neurotoxin Animals Kaempferols Parkinson Disease Secondary Oxidopamine Alpha-synuclein Flavonoids Neurons Multidisciplinary Molecular Structure Plant Extracts Pigments Biological medicine.disease Magnetic Resonance Imaging Astrogliosis Rats Substantia Nigra Disease Models Animal 030104 developmental biology Neuroprotective Agents chemistry Biochemistry alpha-Synuclein Kaempferol 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Safflower has long been used to treat cerebrovascular diseases in China. We previously reported that kaempferol derivatives of safflower can bind DJ-1, a protein associated with Parkinson’s disease (PD) and flavonoid extract of safflower exhibited neuroprotective effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of PD. In this study, a standardized safflower flavonoid extract (SAFE) was isolated from safflower and mainly contained flavonoids. Two marker compounds of SAFE, kaempferol 3-O-rutinoside and anhydrosafflor yellow B, were proven to suppress microtubule destabilization and decreased cell area, respectively. We confirmed that SAFE in dripping pill form could improve behavioural performances in a 6-hydroxydopamine (6-OHDA)-induced rat model of PD, partially via the suppression of α-synuclein overexpression or aggregation, as well as the suppression of reactive astrogliosis. Using an MRI tracer-based method, we found that 6-OHDA could change extracellular space (ECS) diffusion parameters, including a decrease in tortuosity and the rate constant of clearance and an increase in the elimination half-life of the tracer in the 6-OHDA-lesioned substantia nigra. SAFE treatment could partially inhibit the changes in ECS diffusion parameters, which might provide some information about neuronal loss and astrocyte activation. Consequently, our results indicate that SAFE is a potential therapeutic herbal product for treatment of PD. |
| Databáze: | OpenAIRE |
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