An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
| Autor: | Ron M. A. Heeren, Kristine Glunde, Donald F. Smith, Lara Fornai, Erika R. Amstalden van Hove |
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| Rok vydání: | 2011 |
| Předmět: |
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Paper Washing Analyte Surface Properties Proteomics 01 natural sciences Mass spectrometry imaging Mice 03 medical and health sciences Structural Biology Application Note Animals MALDI Spectroscopy 030304 developmental biology Brain Chemistry 0303 health sciences Histocytological Preparation Techniques Chromatography Imaging Mass Spectrometry Chemistry Myocardium 010401 analytical chemistry Proteins Biological tissue Paper based Molecular Imaging Rats 0104 chemical sciences Peptides and Proteins Tissue sections Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Female Molecular imaging Peptides SIMS |
| Zdroj: | Journal of the American Society for Mass Spectrometry |
| ISSN: | 1044-0305 |
| DOI: | 10.1007/s13361-011-0203-z |
| Popis: | Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging. |
| Databáze: | OpenAIRE |
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