Photoreceptor discs form through peripherin-dependent suppression of ciliary ectosome release
| Autor: | Jillian N. Pearring, Vadim Y. Arshavsky, Ying Hao, Jindong Ding, Raquel Y. Salinas, William J. Spencer |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
genetic structures Peripherins Biology Article Mice 03 medical and health sciences 0302 clinical medicine Cell-Derived Microparticles Organelle Extracellular Animals Photoreceptor Cells Cilia Process (anatomy) Research Articles Mice Knockout Cilium Vesicle Peripherin Cell Biology Anatomy Photoreceptor outer segment eye diseases Cell biology Mice Inbred C57BL 030104 developmental biology sense organs 030217 neurology & neurosurgery Function (biology) |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.201608081 |
| Popis: | Visual signal transduction occurs on the surface of membrane discs stacked inside the ciliary outer segment of photoreceptor cells. Salinas et al. show that discs are formed from ciliary ectosomes whose release is blocked by the protein peripherin/RDS. This explains how photoreceptors transform their primary cilia into the light-sensing outer segment organelle. The primary cilium is a highly conserved organelle housing specialized molecules responsible for receiving and processing extracellular signals. A recently discovered property shared across many cilia is the ability to release small vesicles called ectosomes, which are used for exchanging protein and genetic material among cells. In this study, we report a novel role for ciliary ectosomes in building the elaborate photoreceptor outer segment filled with hundreds of tightly packed “disc” membranes. We demonstrate that the photoreceptor cilium has an innate ability to release massive amounts of ectosomes. However, this process is suppressed by the disc-specific protein peripherin, which enables retained ectosomes to be morphed into discs. This new function of peripherin is performed independently from its well-established role in maintaining the high curvature of disc edges, and each function is fulfilled by a separate part of peripherin’s molecule. Our findings explain how the outer segment structure evolved from the primary cilium to provide photoreceptor cells with vast membrane surfaces for efficient light capture. |
| Databáze: | OpenAIRE |
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