Size-exclusion chromatography of procyanidins: Comparison between apple and grape procyanidins and application to the characterization of fractions of high degrees of polymerization
| Autor: | C. Le Bourvellec, Catherine M.G.C. Renard, M. Picot |
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| Přispěvatelé: | Station de Recherches Cidricoles et Biotransformation des Fruits et Légumes (SRC - BFL), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
[CHIM.ANAL] Chemical Sciences/Analytical chemistry Size-exclusion chromatography Fractionation Degree of polymerization MALUS DOMESTICA BORKH 01 natural sciences Biochemistry MATURATION Analytical Chemistry Gel permeation chromatography Acetic acid chemistry.chemical_compound [CHIM.ANAL]Chemical Sciences/Analytical chemistry Environmental Chemistry ComputingMilieux_MISCELLANEOUS Spectroscopy Chromatography Elution fungi 010401 analytical chemistry MOLECULAR WEIGHT 0104 chemical sciences CHIMIE ANALYTIQUE chemistry Polymerization Polyphenol TANNIN 010606 plant biology & botany |
| Zdroj: | Analytica Chimica Acta Analytica Chimica Acta, Elsevier Masson, 2006, 563, pp.33-43 CIÊNCIAVITAE HAL |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2005.06.040 |
| Popis: | Apple procyanidins were fractionated on Toyopearl TSK-gel HW-40S or HW-50S resins eluted by an acetone:6 M urea:acetic acid mixture. Determination of number average degrees of polymerization of the procyanidins in eluted fractions confirmed that in those conditions true molecular weight profiles were obtained. This method was applied to polyphenols extracted from cider apples of different varieties at various stages of maturity, from pear juice and from grape seeds. They confirmed existence of very highly polymerized fractions in perry pears and in certain apple varieties. During post-harvest maturation of apples, the number average degree of polymerization of their procyanidins decreased slightly, and their size-exclusion chromatograms showed disappearance of fractions at low K av values. |
| Databáze: | OpenAIRE |
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