Leukocyte and serum S100A8/S100A9 expression reflects disease activity in ANCA-associated vasculitis and glomerulonephritis
| Autor: | Sally Hamour, S.K. Todd, Paul A. Lyons, Kenneth G. C. Smith, Shaun M. Flint, Charles D. Pusey, H. Terence Cook, Niels Rasmussen, Alan D. Salama, Konstantia-Maria Chavele, Ruth J. Pepper |
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| Přispěvatelé: | Lyons, Paul [0000-0001-7035-8997], Smith, Kenneth [0000-0003-3829-4326], Apollo - University of Cambridge Repository |
| Rok vydání: | 2013 |
| Předmět: |
Male
Systemic disease Pathology Neutrophils Biopsy Kidney vasculitis immunology 0302 clinical medicine fluids and secretions Glomerulonephritis Leukocytes SYSTEMIC-LUPUS-ERYTHEMATOSUS JUVENILE IDIOPATHIC ARTHRITIS Aged 80 and over 0303 health sciences RECEPTOR 4 ANCA Remission Induction Urology & Nephrology Middle Aged Flow Cytometry Prognosis Immunohistochemistry 3. Good health macrophages medicine.anatomical_structure Nephrology Disease Progression Female Inflammation Mediators Vasculitis ANTIBODY-ASSOCIATED VASCULITIS Life Sciences & Biomedicine Adult medicine.medical_specialty Adolescent CD14 Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis Enzyme-Linked Immunosorbent Assay S100A9 S100A8 03 medical and health sciences Young Adult MRP14 Predictive Value of Tests medicine Calgranulin B Humans Calgranulin A Clinical Investigation 030304 developmental biology Aged 030203 arthritis & rheumatology Analysis of Variance Science & Technology business.industry Monocyte REMISSION medicine.disease ENDOTHELIAL-CELLS RHEUMATOID-ARTHRITIS Case-Control Studies Immunology pathology Calprotectin PROTEINS 8 Protein Multimerization business Biomarkers |
| Zdroj: | Kidney International |
| ISSN: | 1523-1755 |
| Popis: | Antineutrophil cytoplasm antibody (ANCA)-associated vasculitis (AAV) commonly results in glomerulonephritis, in which neutrophils and monocytes have important roles. The heterodimer calprotectin (S100A8/S100A9, mrp8/14) is a Toll-like receptor-4 ligand found in neutrophils and monocytes and is elevated in inflammatory conditions. By immunohistochemistry of renal biopsies, patients with focal or crescentic glomerular lesions were found to have the highest expression of calprotectin and those with sclerotic the least. Serum levels of calprotectin as measured by ELISA were elevated in patients with active AAV and the levels decreased but did not normalize during remission, suggesting subclinical inflammation. Calprotectin levels in patients with limited systemic disease increased following treatment withdrawal and were significantly elevated in patients who relapsed compared with those who did not. As assessed by flow cytometry, patients with AAV had higher monocyte and neutrophil cell surface calprotectin expression than healthy controls, but this was not associated with augmented mRNA expression in CD14(+) monocytes or CD16(+) neutrophils. Thus, serum calprotectin is a potential disease biomarker in patients with AAV, and may have a role in disease pathogenesis. |
| Databáze: | OpenAIRE |
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