Single-cell ATAC-Seq reveals cell type-specific transcriptional regulation and unique chromatin accessibility in human spermatogenesis
| Autor: | Sheng Gao, Enkui Duan, Fei Sun, Damin Yun, C. Yan Cheng, Xiaolong Wu, X Wang, Longfei Hu, Mujun Lu, Shitao Chen, Yunhao Wu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Somatic cell ATAC-seq Biology 03 medical and health sciences Meiosis Genetics medicine Humans Spermatogenesis Molecular Biology Transcription factor Genetics (clinical) 0303 health sciences 030305 genetics & heredity General Medicine Chromatin Spermatogonia Cell biology medicine.anatomical_structure CpG site CTCF Chromatin Immunoprecipitation Sequencing Germ cell |
| Zdroj: | Human Molecular Genetics. 31:321-333 |
| ISSN: | 1460-2083 0964-6906 |
| Popis: | During human spermatogenesis, germ cells undergo dynamic changes in chromatin organization/re-packaging and in transcriptomes. In order to better understand the underlying mechanism(s), scATAC-Seq of 5376 testicular cells from 3 normal men were performed. Data were analyzed in parallel with the scRNA-Seq data of human testicular cells. In all, 10 germ cell types associated with spermatogenesis and 6 testicular somatic cell types were identified, along with 142 024 peaks located in promoter, genebody and CpG Island. We had examined chromatin accessibility of all chromosomes, with chromosomes 19 and 17 emerged as the leading chromosomes that displayed high chromatin accessibility. In accessible chromatin regions, transcription factor-binding sites were identified and specific motifs with high frequencies at different spermatogenesis stages were detected, including CTCF, BORIS, NFY, DMRT6, EN1, ISL1 and GLI3. Two most remarkable observations were noted. First, TLE3 was specifically expressed in differentiating spermatogonia. Second, PFN4 was found to be involved in actin cytoskeletal organization during meiosis. More important, unique regions upstream of PFN4 and TLE3 were shown to display high accessibility, illustrating their significance in supporting human spermatogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|