Manganese(II) chloride alters behavioral and neurochemical parameters in larvae and adult zebrafish
| Autor: | Monica R. M. Vianna, Stefani Altenhofen, Laura Roesler Nery, Carlos Eduardo Leite, Melissa Talita Wiprich, Carla Denise Bonan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Nervous system medicine.medical_specialty Programmed cell death Manganese(II) chloride Health Toxicology and Mutagenesis Motor Activity 010501 environmental sciences Aquatic Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neurochemical Chlorides Memory Dopamine Internal medicine Toxicity Tests medicine Animals Zebrafish 0105 earth and related environmental sciences Behavior Animal Cell Death Dose-Response Relationship Drug Tyrosine hydroxylase behavior Dopaminergic Brain Anatomy biology.organism_classification zebrafish Endocrinology medicine.anatomical_structure Manganese Compounds chemistry Larva manganese Female Biomarkers Locomotion Water Pollutants Chemical 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Repositório Institucional PUCRS Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
| Popis: | Manganese (Mn) is an essential metal for organisms, but high levels can cause serious neurological damage. The aim of this study was to evaluate the effects of MnCl 2 exposure on cognition and exploratory behavior in adult and larval zebrafish and correlate these findings with brain accumulation of Mn, overall brain tyrosine hydroxylase (TH) levels, dopamine (DA) levels, 3,4-dihydroxyphenylacetic acid (DOPAC) levels and cell death markers in the nervous system. Adults exposed to MnCl 2 for 4 days (0.5, 1.0 and 1.5 mM) and larvae exposed for 5 days (0.1, 0.25 and 0.5 mM) displayed decreased exploratory behaviors, such as distance traveled and absolute body turn angle, in addition to reduced movement time and an increased number of immobile episodes in larvae. Adults exposed to MnCl 2 for 4 days showed impaired aversive long-term memory in the inhibitory avoidance task. The overall brain TH levels were elevated in adults and larvae evaluated at 5 and 7 days post-fertilization (dpf). Interestingly, the protein level of this enzyme was decreased in larval animals at 10 dpf. Furthermore, DOPAC levels were increased in adult animals exposed to MnCl 2 . Protein analysis showed increased apoptotic markers in both the larvae and adult nervous system. The results demonstrated that prolonged exposure to MnCl 2 leads to locomotor deficits that may be associated with damage caused by this metal in the CNS, particularly in the dopaminergic system. |
| Databáze: | OpenAIRE |
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