Dose response and time course of manganese-enhanced magnetic resonance imaging for visual pathway tracing in vivo
| Autor: | Ying Li, Yun-tao Hu, Zhizhong Ma, Hui Xu, Wei-ling Wang, Xiaodong Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
genetic structures
Neurotransmission optic nerve lcsh:RC346-429 030218 nuclear medicine & medical imaging nerve regeneration manganese 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience In vivo medicine magnetic resonance imaging visual pathway tracing in vivo intravitreal injection time dose neural regeneration lcsh:Neurology. Diseases of the nervous system Retina medicine.diagnostic_test Chemistry Superior colliculus Magnetic resonance imaging Visual cortex medicine.anatomical_structure Axoplasmic transport Optic nerve Neuroscience 030217 neurology & neurosurgery Research Article Biomedical engineering |
| Zdroj: | Neural Regeneration Research, Vol 11, Iss 7, Pp 1185-1190 (2016) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | Axonal tracing is useful for detecting optic nerve injury and regeneration, but many commonly used methods cannot be used to observe axoplasmic flow and synaptic transmission in vivo. Manganese (Mn(2+))-enhanced magnetic resonance imaging (MEMRI) can be used for in vivo longitudinal tracing of the visual pathway. Here, we explored the dose response and time course of an intravitreal injection of MnCl2 for tracing the visual pathway in rabbits in vivo using MEMRI. We found that 2 mM MnCl2 enhanced images of the optic nerve but not the lateral geniculate body or superior colliculus, whereas at all other doses tested (5-40 mM), images of the visual pathway from the retina to the contralateral superior colliculus were significantly enhanced. The images were brightest at 24 hours, and then decreased in brightness until the end of the experiment (7 days). No signal enhancement was observed in the visual cortex at any concentration of MnCl2. These results suggest that MEMRI is a viable method for temporospatial tracing of the visual pathway in vivo. Signal enhancement in MEMRI depends on the dose of MnCl2, and the strongest signals appear 24 hours after intravitreal injection. |
| Databáze: | OpenAIRE |
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