Expression levels of vascular endothelial cell growth factor and microRNA-210 are increased in medulloblastoma and metastatic medulloblastoma
| Autor: | Yong Gao, Puxian Li, Chunyun Song, Zhenrui Liu, Xingtao Diao |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Medulloblastoma
Cancer Research Pathology medicine.medical_specialty Oncogene business.industry Articles General Medicine Cell cycle medulloblastoma medicine.disease vascular endothelial cell growth factor Molecular medicine Primary tumor Metastasis miRNA-210 Immunology and Microbiology (miscellaneous) microRNA Medicine Immunohistochemistry business |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | The present study aimed to investigate the roles of the vascular endothelial cell growth factor (VEGF) and micro (mi)RNA-210 in the metastasis of primary medulloblastoma (MB) tumors. A total of 86 adult patients diagnosed with cerebellar MB were enrolled in the present study, of which 11 patients had metastatic MB in the subarachnoid space. The following samples were collected: MB primary tumor tissue, MB secondary tumor tissue, tumor adjacent tissues and cerebrospinal fluid (CSF). Immunohistochemical analyses of the tissue samples were conducted in order to detect patterns of VEGF expression. In addition, the expression levels of VEGF mRNA and miRNA-210 were analyzed using reverse transcription-quantititative polymerase chain reaction, and western blot analyses were used to investigate VEGF protein expression levels. The positive expression rate of VEGF was significantly higher in MB tumor tissue, as compared with adjacent tissues (P |
| Databáze: | OpenAIRE |
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