Maslinic Acid Enhances Immune Responses in Leukemic Mice Through Macrophage Phagocytosis and Natural Killer Cell Activities In Vivo
| Autor: | Jung Yu Kuo, Chia Chi Liu, Jye Yu Huang, Shu Fen Peng, Rick Sai-Chuen Wu, Jing Gung Chung, Chin Chung Lin, Jr Kai Chen, Zheng Yu Cheng, Kuang Chi Lai |
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| Rok vydání: | 2018 |
| Předmět: |
Cytotoxicity
Immunologic Cancer Research T-Lymphocytes Phagocytosis Spleen Pharmacology Lymphocyte Activation Peripheral blood mononuclear cell General Biochemistry Genetics and Molecular Biology Natural killer cell Mice 03 medical and health sciences 0302 clinical medicine Immune system medicine Splenocyte Animals Humans Macrophage B-Lymphocytes Leukemia Chemistry Macrophages Triterpenes Killer Cells Natural Disease Models Animal medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer cell Leukocytes Mononuclear Research Article |
| Zdroj: | In Vivo. 33:65-73 |
| ISSN: | 1791-7549 0258-851X |
| Popis: | Background/aim Maslinic acid (MA), a pentacyclic triterpene extracted from wax-like coatings of olives, has been shown to reduce cancer cell number through induction of autophagy and apoptosis in many human cancer cells including human leukemia HL-60 cells. In the present study, we investigated whether or not MA affects immune responses in a leukemia mouse model. Materials and methods WEHI-3 cells were intraperitonealIy (i.p.) injected into normal BALB/c mice to develop leukemia. Mice were then treated by i.p. injection with MA at different doses (0, 8, 16 and 32 mg/kg) for 2 weeks. After treatment, all animals were weighed and blood, liver and spleen tissues were weighed. Blood or spleen both were used for determination of cell markers or phagocytosis, natural killer (NK) cell activities and T- and B-cell proliferation, respectively, by using a flow cytometric assay. Results MA did not significantly affect body, liver, and spleen weights. However, MA increased markers of T-cells (at 16 mg/kg treatment) and monocytes (at 32 mg/kg treatment), but reduced B-cell markers (at 8 mg/kg treatment); MA did not significantly affect cell marker of macrophages. Furthermore, MA increased phagocytosis by macrophages from peripheral blood mononuclear cells and peritoneal cavity at 32 mg/kg treatment and increased NK cell activity at target cell:splenocyte ratio of 25:1 but did not affect B- and T-cell proliferation. Conclusion MA increased immune responses by enhancing macrophage phagocytosis and NK cell activities in leukemic mice. |
| Databáze: | OpenAIRE |
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