lyl-1 and tal-1/scl, two genes encoding closely related bHLH transcription factors, display highly overlapping expression patterns during cardiovascular and hematopoietic ontogeny
| Autor: | Dominique Dumenil, Anna-Lila Kaushik, Claude Capron, Sébastien Giroux, Charikleia Kelaidi, Ali Jalil, Isabelle Godin, Olivier Albagli, Fred Sablitzky |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell type Hematopoietic System Embryonic Development In situ hybridization Biology Cardiovascular System Mesoderm Mice Pregnancy Proto-Oncogene Proteins Basic Helix-Loop-Helix Transcription Factors Genetics medicine Animals Molecular Biology Gene Transcription factor In Situ Hybridization T-Cell Acute Lymphocytic Leukemia Protein 1 Messenger RNA Gene Expression Regulation Developmental Hematopoietic stem cell beta-Galactosidase Hematopoiesis Neoplasm Proteins Cell biology Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Female Stem cell Developmental Biology |
| Zdroj: | Gene Expression Patterns. 7:215-226 |
| ISSN: | 1567-133X |
| DOI: | 10.1016/j.modgep.2006.10.004 |
| Popis: | The TAL-1/SCL and LYL-1 genes encode two closely related basic helix-loop-helix transcription factors involved in child T-acute lymphoblastic leukemia through chromosomal rearrangements and transcriptional deregulation. During ontogeny, Tal-1/SCL is required for hematopoietic cell generation, both in the yolk sac, where erythro-myeloid cells are first produced, then in the intra-embryonic compartment, where hematopoietic stem cells independently arise. We describe here the expression pattern of lyl-1 in mouse embryos from 7 to 14 days post coitus using in situ hybridization, as well as beta-Galactosidase (beta-Gal) expression in lyl-1-lacZ knock-in embryos, which express a C-terminally truncated Lyl-1 protein fused to the beta-Galactosidase (Lyl-1Delta/beta-Gal). In addition, we compare lyl-1 expression pattern with that of tal-1/scl. Similar to Tal-1/SCL, Lyl-1 mRNA expression occurs in the developing cardiovascular and hematopoietic systems. However, contrary to tal-1/scl, lyl-1 is not expressed in the developing nervous system. In lyl-1-lacZ knock-in heterozygous and homozygous embryos, beta-Gal expression completely correlates with Lyl-1 mRNA expression in the intra-embryonic compartment and is present: (1) in the developing hematopoietic system, precisely where hematopoietic stem cells emerge, and thereafter in the fetal liver; (2) in the developing vascular system; and (3) in the endocardium. In contrast, whereas Lyl-1 mRNA is expressed in yolk sac-derived endothelial and hematopoietic cells, Lyl-1Delta/beta-Gal is either absent or poorly expressed in these cell types, thus differing from Tal-1/SCL, which is highly expressed there at both mRNA and protein levels. |
| Databáze: | OpenAIRE |
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