A Preadipocyte Differentiation Assay as a Method for Screening Potential Anti-Type II Diabetes Drugs from Herbal Extracts
| Autor: | Yang Ou-yang, Yong-hong Sun, Ming-en Xu, Shang-zhi Xiao, Chen Guan, Xiaoxiang Zheng |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Drug medicine.medical_specialty media_common.quotation_subject Glucose uptake Cell Drug Evaluation Preclinical Gene Expression Pharmaceutical Science Apoptosis Type 2 diabetes Pharmacology Pharmacognosy Analytical Chemistry Rats Sprague-Dawley Rosiglitazone Internal medicine Drug Discovery Gene expression Adipocytes medicine Animals Humans Hypoglycemic Agents Cells Cultured Cell Proliferation media_common Plant Extracts business.industry Organic Chemistry Cell Differentiation medicine.disease Rats PPAR gamma medicine.anatomical_structure Endocrinology Complementary and alternative medicine Molecular Medicine Thiazolidinediones Endothelium Vascular business Drugs Chinese Herbal medicine.drug |
| Zdroj: | Planta Medica. 72:14-19 |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | A cell-based method for screening drug candidates from herbal extracts that have possible anti-type II diabetic effects was established. The differentiation of preadipocytes into adipocytes was used as a sensitive primary indicator of a drug's potential effect on type II diabetes. We established a quantitative method by using a computer image analysis system for assessing the morphological alterations. The assay was validated by screening compounds extracted from Chinese herbs and the known drug rosiglitazone for their capability of modulating PPARgamma gene expression and glucose uptake by adipocytes. Two drug candidates having possible anti-type II diabetic effects were identified. |
| Databáze: | OpenAIRE |
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