A SENSITIVE BIOASSAY OF PARATHYROID HORMONE IN PLASMA
| Autor: | Lucille Bitensky, J. Chayen, J. A. Parsons, Jane Dunham, David J. Chambers, Joan M. Zanelli |
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| Rok vydání: | 1978 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Guinea Pigs Parathyroid hormone Stimulation Glucosephosphate Dehydrogenase In Vitro Techniques Endocrinology Internal medicine medicine Animals Humans Bioassay Kidney Tubules Distal Calcium metabolism Kidney Chemistry Biological activity In vitro Enzyme Activation medicine.anatomical_structure Parathyroid Hormone Biological Assay Female Hormone |
| Zdroj: | Clinical Endocrinology. 9:375-379 |
| ISSN: | 1365-2265 0300-0664 |
| Popis: | SUMMARY Understanding of calcium metabolism in health and disease has been retarded by the lack of an adequately sensitive bioassay of parathyroid hormone. The problem of dissociation of bioactivity and immunoactivity, well recognized for other polypeptide hormones, is exaggerated in the case of parathyroid hormone by the disproportionately long half-time in the circulation of the immunoreactive fragments. A new method of assaying the biological activity of parathyroid hormone in plasma has been developed, based on the cytochemical methods which have yielded highly sensitive bioassays of other polypeptide hormones. It depends on the stimulation of glucose 6-phosphate dehydrogenase activity in the distal convoluted tubules of segments of guinea-pig kidney maintained in vitro, and measured by microdensitometry. The limit of sensitivity of the assay is 5 fg/ml (bPTH); the index of precision is 0.09 ± 0.04 (mean ± SEM; n= 11). |
| Databáze: | OpenAIRE |
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