Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect nonrecombinants
| Autor: | J.C. Fuscoe |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Genetics
Base Sequence Library DNA Recombinant Nucleic Acid Hybridization Chromosome DNA General Medicine Biology Molecular biology Insert (molecular biology) law.invention chemistry.chemical_compound Oligodeoxyribonucleotides chemistry law Cloning Site Recombinant DNA Chromosomes Human Humans Genomic library Molecular probe Information Systems |
| Zdroj: | Gene. 52:291-296 |
| ISSN: | 0378-1119 |
| Popis: | The frequencies of non-recombinants are directly determined in 21 human DNA libraries prepared from flow-purified chromosomes. A radiolabeled 18-base oligodeoxynucleotide, whose sequence spans the cloning site, stably hybridizes to non-recombinants. When the cloning site is interrupted by an insert, less stable hybridization occurs. Non-recombinant frequencies range from 1–34% with the frequency of false-positives being less than 1 %. Libraries are being prepared for each human chromosome as part of the National Laboratory Gene Library Project and the specifications of seven new libraries are presented. |
| Databáze: | OpenAIRE |
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