Use of degenerate oligonucleotide primed polymerase chain reaction for detection of chicken anaemia virus contamination in avian viral vaccines
| Autor: | S-E. Tabatabaeizadeh, M. Naeemipour, M. Jamshidian-Mojaver, H. R. Farzin, M. R. Bassami |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures lcsh:Veterinary medicine General Veterinary biology 040301 veterinary sciences Viral Vaccine viruses 04 agricultural and veterinary sciences chicken anaemia virus (cav) Contamination Degenerate oligonucleotide biology.organism_classification Virology law.invention 0403 veterinary science 03 medical and health sciences 030104 developmental biology law cardiovascular system lcsh:SF600-1100 Chicken anaemia virus avian viral vaccines quality control Polymerase chain reaction |
| Zdroj: | Bulgarian Journal of Veterinary Medicine, Vol 23, Iss 3, Pp 304-309 (2020) |
| ISSN: | 1313-3543 1311-1477 |
| Popis: | For quality control of biologicals of veterinary use, the absence of extraneous agents needs to be certified. One of the requirements for quality control of avian viral vaccines is to demonstrate freedom from extraneous and adventitious pathogenic agents, like chicken anaemia virus (CAV). In this study, a degenerate oligonucleotide primed PCR (DOP-PCR) for the detection of CAV was developed. Degenerate oligonucleotide primers were selected based on sequences corresponding to conserved regions of VP1 gene. After spiking of CAV genomic DNA to an infectious laryngotracheitis virus (ILTV) vaccine, detection limit for the test was 3.056×10-9 ng/µl. To evaluate the performance of the test, 11 avian viral vaccines including infectious bronchitis virus (IBV), newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and ILTV vaccines from 5 manufacturers were screened for CAV and no contamination was detected. The test described here may provide a rapid, sensitive and specific method for contamination detection of avian viral vaccines with CAV, and may be applied for quality control of live and killed commercial vaccines. |
| Databáze: | OpenAIRE |
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