Virus-host coexistence in phytoplankton through the genomic lens
| Autor: | Jean-Marc Aury, Marie-Line Escande, Yves Van de Peer, Gwenael Piganeau, Emmelien Vancaester, Sheree Yau, Julie Guy, Mathieu Groussin, Sophie Sanchez-Brosseau, L. Felipe Benites, Nigel Grimsley, Klaas Vandepoele, Sébastien Gourbière, Hervé Moreau, Stephane Rombauts, Evelyne Derelle, Yves Desdevises, Marc Krasovec |
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| Přispěvatelé: | Biologie intégrative des organismes marins (BIOM), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institute of Marine Sciences / Institut de Ciències del Mar [Barcelona] (ICM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Universiteit Gent = Ghent University [Belgium] (UGENT), VIB [Belgium], Massachusetts Institute of Technology (MIT), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Pretoria [South Africa], Laboratoire Génome et développement des plantes (LGDP), Université de Perpignan Via Domitia (UPVD)-Centre National de la Recherche Scientifique (CNRS), ANR-12-BSV7-0006,REVIREC,Résistance aux prasinovirus analysée par la transcriptomique et la recombinaison(2012), ANR-12-BSV7-0009,DECOVIR,Diversité des prasinovirus et contrôle par les facteurs environnementaux(2012), ANR-13-JSV6-0005,PHYTNESS,Mutation, Recombinaison et Fitness des Eucaryotes, Approches Génomiques sur les Microalgues du Phytoplankton(2013), European Project: 675752,H2020,H2020-MSCA-ITN-2015,SINGEK(2016), Agence Nationale de la Recherche (France), European Commission, Agencia Estatal de Investigación (España), Universiteit Gent = Ghent University (UGENT), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO) |
| Rok vydání: | 2020 |
| Předmět: |
DYNAMICS
[SDV]Life Sciences [q-bio] viruses Population DIVERSITY QUANTITATIVE PCR Oceanography Microbiology SEQUENCE Virus Ostreococcus tauri 03 medical and health sciences Microalgae MODE Symbiosis education Research Articles EUKARYOTE 030304 developmental biology Comparative genomics Evolutionary Biology 0303 health sciences education.field_of_study Experimental evolution Genome Multidisciplinary OSTREOCOCCUS-TAURI biology Strain (chemistry) ACQUISITION 030306 microbiology ACL SciAdv r-articles Biology and Life Sciences Chromosome Genomics Models Theoretical biology.organism_classification Evolutionary biology Host-Pathogen Interactions Phytoplankton Eukaryote [SDE.BE]Environmental Sciences/Biodiversity and Ecology MARINE Algorithms RESISTANCE Research Article |
| Zdroj: | Science Advances (2375-2548) (American Association for the Advancement of Science (AAAS)), 2020-04, Vol. 6, N. 14, P. eaay2587 (12p.) Science Advances Science Advances, American Association for the Advancement of Science (AAAS), 2020, 6 (14), pp.eaay2587. ⟨10.1126/sciadv.aay2587⟩ SCIENCE ADVANCES Digital.CSIC. Repositorio Institucional del CSIC instname Science Advances, 2020, 6 (14), pp.eaay2587. ⟨10.1126/sciadv.aay2587⟩ |
| ISSN: | 2375-2548 0000-0000 |
| Popis: | 11 pages, 5 figures, 1 table, supplementary materials http://advances.sciencemag.org/cgi/content/full/6/14/eaay2587/DC1.-- Data and materials availability: The genome sequence for O. mediterraneus RCC2590 and OmV2 can be found under GenBank accession numbers WMKK00000000 and MN688676, respectively. The complete gene annotations of RCC2590 can be accessed on ORCAE (https://bioinformatics.psb.ugent.be/orcae/). MMETSP sequence data can be accessed from www.imicrobe.us/#/projects/104. The genomes and their GenBank accession numbers used in this study are as follows: B. prasinos (GCA_002220235.1), M. commoda RCC299 (GCA_000090985.2), M. pusilla CCMP1545 (GCA_000151265.1), O. tauri RCC4221 (CAID01000001.2 to CAID01000020.2, CR954200.2, and CR954199.2), O. lucimarinus CCE9901 (GCA_000092065.1), BpV1 (HM004432.1), BpV2 (HM004430.1), MpV1 (HM004429.1), OtV1 (FN386611.1), OtV2 (FN600414.1), OtV5 (EU304328.2), OtV6 (JN225873.1), OlV1 (HM004431.1), OlV2 (KP874736.1), OlV3 (HQ633060.1), OlV4 (JF974316.1), OlV5 (HQ632827.1), OlV6 (HQ633059.1), OlV7 (KP874737.1), and OmV1 (KP874735.1). Ostreococcus sp. RCC809 is available from the JGI Genome Portal under project ID 16233 Virus-microbe interactions in the ocean are commonly described by “boom and bust”dynamics, whereby a numerically dominant microorganism is lysed and replaced by a virus-resistant one. Here, we isolated a microalga strain and its infective dsDNA virus whose dynamics are characterized instead by parallel growth of both the microalga and the virus. Experimental evolution of clonal lines revealed that this viral production originates from the lysis of a minority of virus-susceptible cells, which are regenerated from resistant cells. Whole-genome sequencing demonstrated that this resistant-susceptible switch involved a large deletion on one chromosome. Mathematical modeling explained how the switch maintains stable microalga-virus population dynamics consistent with their observed growth pattern. Comparative genomics confirmed an ancient origin of this “accordion” chromosome despite a lack of sequence conservation. Together, our results show how dynamic genomic rearrangements may account for a previously overlooked coexistence mechanism in microalgae-virus interactions. This research was funded by the ANR grants REVIREC ANR-12-BSV7-0006-01, DECOVIR ANR-12-BSV7-0009, and PHYTNESS ANR-13-JSV6-0005. E.V. is funded by BOF project GOA 01G01715, and L.F.B. is funded by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. H2020-MSCA-ITN-2015-675752 With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) |
| Databáze: | OpenAIRE |
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