Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein
| Autor: | Pasquale Piazzolla, Angela Ostuni, A. De Stradis, Maria Nuzzaci, Alfonso Bavoso, Aniello Crescenzi, Soccorsa Comes, L. D'Aquino |
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| Rok vydání: | 1997 |
| Předmět: |
genetic structures
Blotting Western Molecular Sequence Data Potyvirus PPV Enzyme-Linked Immunosorbent Assay Peptide Antibodies Viral IMMUNOLOGY Capsid Species Specificity Virology SYNTHETIC PEPTIDES Amino Acid Sequence Cloning Molecular Microscopy Immunoelectron Peptide sequence Plant Diseases Antiserum chemistry.chemical_classification biology Strain (chemistry) Plant Extracts Nucleic acid sequence biology.organism_classification Molecular biology Peptide Fragments ELECTRON MICROSCOPY chemistry Polyclonal antibodies biology.protein Capsid Proteins Sequence Analysis |
| Zdroj: | Journal of virological methods 69 (1997): 181–189. doi:10.1016/S0166-0934(97)00158-4 info:cnr-pdr/source/autori:A. Crescenzi; L. d'Aquino; M. Nuzzaci; A. Ostuni; A. Bavoso; S. Comes; De Stradis A; P. Piazzolla/titolo:Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat Protein/doi:10.1016%2FS0166-0934(97)00158-4/rivista:Journal of virological methods/anno:1997/pagina_da:181/pagina_a:189/intervallo_pagine:181–189/volume:69 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(97)00158-4 |
| Popis: | Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests. |
| Databáze: | OpenAIRE |
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