In vivo quantification of the secretion rates of the hemolysin A Type I secretion system
| Autor: | Sander H. J. Smits, Michael H. H. Lenders, Tobias Beer, Lutz Schmitt |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Folding ATP-binding cassette transporter Biology Hemolysin Proteins Article 03 medical and health sciences Escherichia coli Extracellular Secretion chemistry.chemical_classification Type I Secretion Systems Multidisciplinary 030102 biochemistry & molecular biology Membrane fusion protein Calcium-Binding Proteins Biological Transport Amino acid Cell biology 030104 developmental biology Biochemistry chemistry ATP-Binding Cassette Transporters Calcium Protein folding Bacterial outer membrane |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep33275 |
| Popis: | Type 1 secretion systems (T1SS) of Gram-negative bacteria secrete a broad range of substrates into the extracellular space. Common to all substrates is a C-terminal secretion sequence and nonapeptide repeats in the C-terminal part that bind Ca2+ in the extracellular space, to trigger protein folding. Like all T1SS, the hemolysin A (HlyA) T1SS of Escherichia coli consists of an ABC transporter, a membrane fusion protein and an outer membrane protein allowing the one step translocation of the substrate across both membranes. Here, we analyzed the secretion rate of the HlyA T1SS. Our results demonstrate that the rate is independent of substrate-size and operates at a speed of approximately 16 amino acids per transporter per second. We also demonstrate that the rate is independent of the extracellular Ca2+ concentration raising the question of the driving force of substrate secretion by T1SS in general. |
| Databáze: | OpenAIRE |
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