A Middle Cerebral Artery Occlusion Technique for Inducing Post-stroke Depression in Rats
| Autor: | Honore N Shiyntum, Ruslan Kuts, Evgeni Brotfain, Alexander Zlotnik, Dmitri Frank, Julia Grinshpun, Matthew Boyko, Israel Melamed, Michael Dubilet, Dmitry Natanel |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Middle Cerebral Artery General Chemical Engineering External carotid artery Infarction Neuroprotection General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley 03 medical and health sciences Food Preferences 0302 clinical medicine Internal medicine medicine.artery Medicine Post-stroke depression Animals cardiovascular diseases Depression (differential diagnoses) Swimming General Immunology and Microbiology business.industry Depression General Neuroscience Infarction Middle Cerebral Artery medicine.disease Pathophysiology Rats Disease Models Animal 030104 developmental biology nervous system Middle cerebral artery Cardiology Internal carotid artery business Carotid Artery Injuries 030217 neurology & neurosurgery |
| Zdroj: | Journal of visualized experiments : JoVE. (147) |
| ISSN: | 1940-087X |
| Popis: | Post-stroke depression (PSD) is the most recurrent of all psychiatric complications resulting from an ischemic stroke. A greater majority (about 60%) of all ischemic stroke patients suffer from PSD, a disorder considered to be an ischemic stroke-related precursor for increased death and degradation in health. The pathophysiology of PSD is still obscure. To study the mechanism of development and occurrence of PSD further, and to find out a therapy, we attempted to develop a new protocol that requires occluding the middle cerebral artery (MCA) via the internal carotid artery (ICA) in rats. This protocol describes a model of PSD induced in rats through the middle cerebral artery occlusion (MCAO). Also used in the experiment are the Porsolt forced swim test and the sucrose preference test to confirm and evaluate the depressive mood of the rats under investigation. Rather than inserting the catheter through the external carotid artery (ECA), as stipulated for the original procedure, this MCAO technique has the monofilament passing directly through the ICA. This MCAO technique was developed a few years ago and leads to a reduction in mortality and variability. It is generally accepted that the criteria used are preferred in the selection of biological models. The data obtained with this protocol show that this model of MCAO could be a way of inducing PSD in rats and could potentially lead to the understanding of the pathophysiology and the future development of new drugs and other neuroprotective agents. |
| Databáze: | OpenAIRE |
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