Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
| Autor: | Man-Man Ning, Shuang Li, Siyu Pei, Shaoqing Tang, Shangli Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Camellia huana
0106 biological sciences Genetic diversity biology Haplotype Endangered species Plant Science biology.organism_classification 010603 evolutionary biology 01 natural sciences lcsh:QK1-989 lcsh:Biology (General) Chloroplast DNA Evolutionary biology lcsh:Botany Genetic structure Microsatellite Conservation implications Theaceae Allele lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Plant Diversity, Vol 42, Iss 5, Pp 343-350 (2020) |
| ISSN: | 2468-2659 |
| DOI: | 10.1016/j.pld.2020.06.003 |
| Popis: | Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively. |
| Databáze: | OpenAIRE |
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