In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model
| Autor: | Seung Ha Park, Eun Sang Dhong, Seung Chul Rhee, Eul Sik Yoon, Yi Hwa Ji, Nareg A. Gharibjanian |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Bone Regeneration Bone density Adipose tissue Bone Matrix Mice Nude Biology Rats Sprague-Dawley Mice Random Allocation Implants Experimental Antigens CD Bone Density medicine Animals Hedgehog Proteins Bone regeneration Bone Demineralization Technique Osteoblasts Tissue Scaffolds Demineralized bone matrix dBm Skull Veratrum Alkaloids Cell Differentiation Cell Biology Hematology Anatomy Stromal vascular fraction Antigens Differentiation Rats Adipose Tissue Stem cell Stromal Cells Developmental Biology Signal Transduction Stem Cell Transplantation |
| Zdroj: | Stem cells and development. 20(2) |
| ISSN: | 1557-8534 |
| Popis: | Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX(®)) and/or poly(70L-lactide-co-30DL-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM + SVF, (4) DBM + PLA, and (5) DBM + PLA + SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM + SVF and DBM + PLA + SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.0300.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM + SVF: 57.69%; DBM + PLA: 24.86%; DBM + PLA + SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM + SVF and DBM + PLA + SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy. |
| Databáze: | OpenAIRE |
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