Achieving the balance: Biphasic effects of genistein on PC-3 cells

Autor: Beyza Goncu, Nur Ozten‐Kandas, Sule Terzioglu-Usak, Mehmet Yildiz
Přispěvatelé: ÖZTEN KANDAŞ, NUR
Rok vydání: 2019
Předmět:
Male
030309 nutrition & dietetics
Cell Survival
Cell
Biophysics
Genistein
Apoptosis
Smad Proteins
SMAD
Pharmacology
03 medical and health sciences
Prostate cancer
chemistry.chemical_compound
0404 agricultural biotechnology
In vivo
Transforming Growth Factor beta
medicine
Humans
Cell Proliferation
0303 health sciences
Chemistry
Cancer
Prostatic Neoplasms
04 agricultural and veterinary sciences
Cell Biology
Biphasic effects of genistein on PC-3 cells-
JOURNAL OF FOOD BIOCHEMISTRY
cilt.129
ss.1-10
2019 [TERZİOĞLU Ş.
Yildiz M. T.
Goncu B.
Ozten Kandas N.
-Achieving the balance]
medicine.disease
040401 food science
medicine.anatomical_structure
Mechanism of action
PC-3 Cells
medicine.symptom
Biphasic effects of genistein on PC-3 cells-
JOURNAL OF FOOD BIOCHEMISTRY
cilt.43
2019 [Terzioglu-Usak S.
Yildiz M. T.
Goncu B.
Ozten-Kandas N.
-Achieving the balance]
Food Science
Transforming growth factor
DNA Damage
Zdroj: Journal of food biochemistry. 43(8)
ISSN: 1745-4514
Popis: This study examined the response of PC-3 cells to physiological (0.5, 2.5, 5, 10 μM) and pharmacological (50 μM) concentrations of genistein which is a main bioactive compound in soy. Following 48 hr genistein treatment, cell-based assays and genome-wide microarray were performed. It was evidenced that maximal physiologically achievable concentrations of genistein (0.5-10 μM) lead to significant increase in cell viability (p 0.05) and decrease in migration at 0.5 μM (p = 0.000) and 10 μM (p = 0.001). The highest percentage of apoptotic cells was obtained at 50 μM. Microarray analysis gave the most critical pathways such as cell cycle regulation and proliferation, tumorigenesis, DNA damage and repair, stress response, and apoptosis. Physiological concentrations (≤10 μM) induced activation of CDKs, MAPKs, and RPSKs, while high concentrations of genistein (10 μM) appeared to have a novel mechanism of action, specifically down-regulating TGF-β by decreasing specifically SMAD 2/3,4 which are in the downstream TGF-β signaling cascade. PRACTICAL APPLICATIONS: This study highlights for the first time that maximal physiologically achievable concentrations of genistein (0.5-10 μM) have proliferative effects evidenced by alterations in global gene expression patterns of PC-3 cells. Our results particularly represent a closer examination of dietary genistein consumption for the prevention and/or treatment of cancer that maximal physiologically achievable concentrations of genistein could have detrimental effects on individuals with prostate cancer. Further studies as in vivo would be necessary to remove shadows on the effect of genistein on prostate cancer progression.
Databáze: OpenAIRE
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