High-Resolution Melting Analysis in Comparison with Microscopic Method: An Experimental Study to Diagnosis of Plasmodium Species Infections in Human
| Autor: | Mehdi Nateghpour, Sina Mohtasebi, Mehdi Mohebali, Mohammad Javad Abbaszadeh Afshar, Ahmad Raeisi, Mahya Allahmoradi, Afsaneh Motevalli Haghi, Ahmad Hosseini Safa |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Plasmodium 030231 tropical medicine High Resolution Melt lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Malaria elimination Internal medicine parasitic diseases Diagnosis medicine Microscopic method lcsh:RC109-216 030212 general & internal medicine Plasmodium species Microscopy biology business.industry Plasmodium falciparum medicine.disease biology.organism_classification Malaria Infectious Diseases Original Article Parasitology National laboratory business |
| Zdroj: | Iranian Journal of Parasitology, Vol 15, Iss 3 (2020) Iranian Journal of Parasitology |
| ISSN: | 1735-7020 |
| Popis: | Background: Among the human parasitic diseases, malaria is the main cause of morbidity and mortality. To prevent the high mortality and tracking malaria elimination efforts, a prompt and sensitive diagnosis is essential. This study aimed to compare High-Resolution Melting (HRM) and microscopic methods to diagnose Plasmodium falciparum and P. vivax. Methods: Eighty-one blood samples were collected from patients with clinical symptoms who were suspect to malaria in Chabahar district, southeastern Iran and also, from those who were referred to Malaria National Laboratory in the Tehran University of Medical Sciences, Tehran, Iran. Microscopic examination and HRM method were used to the diagnosis of Plasmodium parasites simultaneously. Results: Microscopic results revealed 45 positive cases (12 P. falciparum and 33 P. vivax) out of 81 collected samples while according to HRM analysis results 11 and 33 samples were identified as P. falciparum and P. vivax, respectively. HRM analysis also revealed 1 mixed infection of P. falciparum and P. malariae. Conclusion: HRM analysis provides a promising mean for simultaneous detection and discrimination of the Plasmodium spp. especially in mixed infection cases. |
| Databáze: | OpenAIRE |
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