Reprint of: Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities
| Autor: | Jamie Grifo, Mina Alikani, Santiago Munné, Giles Tomkin, Jacques Cohen |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures 030219 obstetrics & reproductive medicine In vitro fertilisation medicine.diagnostic_test medicine.medical_treatment Obstetrics and Gynecology Aneuploidy Prenatal diagnosis Biology medicine.disease Preimplantation genetic diagnosis Molecular biology Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine embryonic structures medicine Advanced maternal age Ploidy Trisomy Fluorescence in situ hybridization |
| Zdroj: | Fertility and Sterility. 112:e71-e80 |
| ISSN: | 0015-0282 |
| Popis: | Objective To determine some of the unresolved questions related to chromosome anomalies in early human embryos, such are the detection of any advanced maternal age effect; the complete assessment of mosaicism, which requires analysis of all cells; and the relationship with embryonic dysmorphism. Fluorescence in situ hybridization has been used in this study to answer these issues. Design Fluorescence in situ hybridization analysis of human embryos using simultaneously probes for three or five chromosomes. Five hundred twenty-four cleavage-stage human embryos obtained by IVF were analyzed by fluorescence in situ hybridization. Embryos were allocated into three groups according to morphological and developmental characteristics (arrested; slow and/or fragmented; morphologically and developmentally normal). The embryos also were analyzed according to maternal age. Results Dysmorphic embryos had higher rates of polyploidy and diploid mosaicism. Aneuploidy increased with maternal age in nonarrested embryos. Preimplantation genetic diagnosis successfully detected these abnormalities. Conclusion This study demonstrates that, in morphologically and developmentally normal human embryos, cleavage-stage aneuploidy significantly increases with maternal age. The results suggest that implantation failure in older women largely could be due to aneuploidy. |
| Databáze: | OpenAIRE |
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