Study on the effects of gradient mechanical pressures on the proliferation, apoptosis, chondrogenesis and hypertrophy of mandibular condylar chondrocytes in vitro
| Autor: | Hui Li, Linjian Huang, Chi Yang, Shaoyi Wang, Qianyang Xie, Min Zhang, Xieyi Cai |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Necrosis Apoptosis Chondrocyte hypertrophy Cell Enlargement Chondrocyte Muscle hypertrophy 03 medical and health sciences Chondrocytes 0302 clinical medicine Internal medicine Pressure medicine Animals Aggrecans General Dentistry Aggrecan Cell Proliferation Chemistry Cartilage Mandibular Condyle Cell Differentiation Hypertrophy 030206 dentistry Cell Biology General Medicine Alkaline Phosphatase Chondrogenesis respiratory tract diseases Cell biology 030104 developmental biology medicine.anatomical_structure Endocrinology Otorhinolaryngology Collagen Rabbits medicine.symptom |
| Zdroj: | Archives of Oral Biology. 73:186-192 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2016.10.017 |
| Popis: | Objective To investigate the effects of gradient mechanical pressure on chondrocyte proliferation, apoptosis, and the expression of markers of chondrogenesis and chondrocyte hypertrophy. Methods Mandibular condylar chondrocytes from 5 rabbits were cultured in vitro, and pressed with static pressures of 50 kPa, 100 kPa, 150 kPa and 200 kPa for 3 h, respectively. The chondrocytes cultured without pressure (0 kPa) were used as control. Cell proliferation, apoptosis, and the expression of aggrecan (AGG), collagen II (COL2), collagen X (COL10), alkaline phosphatase (ALP) were investigated. Ultrastructures of the pressurized chondrocytes under transmission electron microscopy (TEM) were observed. Results Chondrocyte proliferation increased at 100 kPa and decreased at 200 kPa. Chondrocyte apoptosis increased with peak pressure at 200 kPa in a dose-dependent manner. Chondrocyte necrosis increased at 200 kPa. The expression of AGG increased at 200 kPa. The expression of COL2 decreased at 50 kPa and increased at 150 kPa. The expression of COL10 and ALP increased at 150 kPa. Ultrastructure of the pressurized chondrocytes under TEM showed: at 100 kPa, cells were enlarged with less cellular microvillus and a bigger nucleus; at 200 kPa, cells shrank with the sign of apoptosis, and apoptosis cells were found. Conclusions The mechanical loading of 150 kPa is the moderate pressure for chondrocyte: cell proliferation and apoptosis is balanced, necrosis is reduced, and chondrogenesis and chondrocyte hypertrophy are promoted. When the pressure is lower, chondrogenesis and chondrocyte hypertrophy are inhibited. At 200 kPa, degeneration of cartilage is implied. |
| Databáze: | OpenAIRE |
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