Analyzing Defects in the Caenorhabditis elegans Nervous System Using Organismal and Cell Biological Approaches
| Autor: | Megan Guziewicz, Bethany Simmons, Toni Vitullo, Rebecca Kohn |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nervous system
Movement Green Fluorescent Proteins Membrane Fusion Nervous System Synaptic vesicle Education Green fluorescent protein chemistry.chemical_compound Education Professional medicine Animals Nervous System Physiological Phenomena Caenorhabditis elegans Neurotransmitter Biology biology Articles Cell Biology biology.organism_classification Cell biology Vesicular transport protein Luminescent Proteins medicine.anatomical_structure Microscopy Fluorescence chemistry Synaptic vesicle transport Synaptic Vesicles Laboratories Neuroscience Function (biology) |
| Zdroj: | Cell Biology Education. 1:18-25 |
| ISSN: | 1536-7509 |
| DOI: | 10.1187/cbe.01-08-0001 |
| Popis: | The goal of this laboratory exercise is to increase student understanding of the impact of nervous system function at both the organismal and cellular levels. This inquiry-based exercise is designed for an undergraduate course examining principles of cell biology. After observing the movement of Caenorhabditis elegans with defects in their nervous system, students examine the structure of the nervous system to categorize the type of defect. They distinguish between defects in synaptic vesicle transport and defects in synaptic vesicle fusion with membranes. The synaptic vesicles are tagged with green fluorescent protein (GFP), simplifying cellular analysis. The expected outcome of this experiment is that students will better understand the concepts of vesicle transport, neurotransmitter release, GFP, and the relation between the nervous system and behavior. |
| Databáze: | OpenAIRE |
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