An enzymatic assay of inorganic phosphate in serum using nucleoside phosphorylase and xanthine oxidase
| Autor: | S. H. Bissbort, Jacobus P.J. Ungerer, Heila M. Oosthuizen |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Xanthine Oxidase Chromatography Biochemistry (medical) Clinical Biochemistry Purine nucleoside phosphorylase General Medicine Xanthine Phosphate Biochemistry Phosphates chemistry.chemical_compound Enzyme chemistry Spectrophotometry medicine Humans Uric acid Pentosyltransferases Xanthine oxidase Inosine Oxidation-Reduction Hypoxanthine medicine.drug |
| Zdroj: | Clinica Chimica Acta. 223:148-157 |
| ISSN: | 0009-8981 |
| Popis: | We have developed a new enzymatic assay for the determination of inorganic phosphate (Pi) in serum, using nucleoside phosphorylase (NP) and xanthine oxidase (XOD). Pi and inosine react to form hypoxanthine and ribose-1-phosphate. The hypoxanthine is oxidized to xanthine, which is further oxidized to uric acid. In these two reactions 2,6-dichlorophenol-indophenol (DCIP) is reduced to a colourless compound and the decrease in colour is measured spectrophotometrically at 600 nm. The assay is automated with an RA-XT analyser. The precision of the automated assay is acceptable (C.V.3.5%) and results are accurate and linear across a range of values from 0.2-2.5 mmol/l. The assay correlates well with molybdate methods carried out on SMAC III and RA-XT analysers (r values 0.99 and 0.98, respectively), and seems to be less prone to non-specific sample interference than the usual RA-XT method. The enzymatic assay described seems to be suitable for the routine determination of serum Pi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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