'Omics' of Food-Borne Gastroenteritis: Global Proteomic and Mutagenic Analysis ofSalmonella entericaSerovar Enteritidis
| Autor: | Aryashree Arunima, Bhawna Gupta, Chandrashekhar Padhi, Sangeeta Jaiswal, Daniel Ryan, Jayshree Advani, Harsha Gowda, Soujanya D. Yelamanchi, Gajanan Sathe, Mrutyunjay Suar, T. S. Keshava Prasad |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine RNA Untranslated Virulence Factors Protein subunit Salmonella enteritidis 030106 microbiology Hypothetical protein Mutant Biology Biochemistry Cell Line Microbiology Mice 03 medical and health sciences Bacterial Proteins RNA Transfer Genetics Animals Secretion Molecular Biology Virulence biochemical phenomena metabolism and nutrition Gastroenteritis Intestines RAW 264.7 Cells Membrane protein Food Salmonella Infections Transfer RNA Molecular Medicine Alpha chain Mutagens Biotechnology |
| Zdroj: | OMICS: A Journal of Integrative Biology. 21:571-583 |
| ISSN: | 1557-8100 |
| Popis: | Salmonella Enteritidis causes food-borne gastroenteritis by the two type three secretion systems (TTSS). TTSS-1 mediates invasion through intestinal lining, and TTSS-2 facilitates phagocytic survival. The pathogens' ability to infect effectively under TTSS-1-deficient background in host's phagocytes is poorly understood. Therefore, pathobiological understanding of TTSS-1-defective nontyphoidal Salmonellosis is highly important. We performed a comparative global proteomic analysis of the isogenic TTSS-1 mutant of Salmonella Enteritidis (M1511) and its wild-type isolate P125109. Our results showed 43 proteins were differentially expressed. Functional annotation further revealed that differentially expressed proteins belong to pathogenesis, tRNA and ncRNA metabolic processes. Three proteins, tryptophan subunit alpha chain, citrate lyase subunit alpha, and hypothetical protein 3202, were selected for in vitro analysis based on their functional annotations. Deletion mutants generated for the above proteins in the M1511 strain showed reduced intracellular survival inside macrophages in vitro. In sum, this study provides mass spectrometry-based evidence for seven hypothetical proteins, which will be subject of future investigations. Our study identifies proteins influencing virulence of Salmonella in the host. The study complements and further strengthens previously published research on proteins involved in enteropathogenesis of Salmonella and extends their role in noninvasive Salmonellosis. |
| Databáze: | OpenAIRE |
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