Perfil protéico como indicador qualitativo do semen criopreservado de Tambaqui Colossoma macropomum (Cuvier, 1818)
| Autor: | J. M. Galo, D. P. Streit-Jr, C. D. Corcini, A. S. Varela-Jr, R. D. Jardim, R. P. Ribeiro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
fertilization rate QH301-705.5 Science Tambaqui Semen Protein profile Biology cryopreservation Cryopreservation Andrology Human fertilization Animals Humans spermatic motility Biology (General) Sperm quality motilidade espermática Colossoma macropomum proteínas criopreservação Botany 0402 animal and dairy science Proteins 04 agricultural and veterinary sciences Straw biology.organism_classification 040201 dairy & animal science Spermatozoa proteins QL1-991 QK1-989 040102 fisheries Sperm Motility 0401 agriculture forestry and fisheries General Agricultural and Biological Sciences Zoology taxa de fertilização Semen Preservation |
| Zdroj: | Brazilian Journal of Biology, Issue: ahead, Published: 25 NOV 2019 Brazilian Journal of Biology v.80 n.4 2020 Brazilian Journal of Biology Instituto Internacional de Ecologia (IIE) instacron:IIE Brazilian Journal of Biology, Volume: 80, Issue: 4, Pages: 752-762, Published: 25 NOV 2019 Brazilian Journal of Biology, Vol 80, Iss 4, Pp 752-762 (2019) |
| Popis: | The aim of this study was to evaluate the association between proteins in the seminal plasma of tambaqui Colossoma macropomum (Cuvier, 1818) with seminal quality indicators after thawing. The semen was cryopreserved with a dilution based on BTS with 8% DMSO. A 200 µL sample of semen from each animal was diluted in 800 µL BTS, centrifuged at 800 rpm, and the supernatant was cryopreserved to further analyze of the protein profile of seminal plasma through one-dimensional electrophoresis (SDS-PAGE). After 15 days of cryopreservation, a cryopreserved semen straw was thawed to analyze both qualitative and quantitative parameters. When considering all collections, the SDS-PAGE identified 15 protein bands in the seminal plasma of tambaqui. When the interaction (presence or absence) between proteins observed in the seminal plasma and the post thawed spermatic parameters was evaluated, we observed a great influence of the presence of proteins on spermatic quality. A greater (P |
| Databáze: | OpenAIRE |
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