Modulation of Th2 type cytokine production from human peripheral blood leukocytes by a macrolide antibiotic, roxithromycin, in vitro
| Autor: | Tadashi Hisamitsu, Kazuhito Asano, Harumi Suzaki, Kiyoaki Kamakazu |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male medicine.medical_treatment Immunology Enzyme-Linked Immunosorbent Assay In Vitro Techniques Th2 Cells Interferon Leukocytes Humans Immunology and Allergy Medicine Interferon gamma Antibacterial agent Pharmacology Roxithromycin business.industry Antibodies Monoclonal Rhinitis Allergic Seasonal Interleukin T lymphocyte Middle Aged Th1 Cells Stimulation Chemical In vitro Anti-Bacterial Agents Cytokine Cytokines Female business Cell Division medicine.drug |
| Zdroj: | International Immunopharmacology. 1:1913-1921 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/s1567-5769(01)00116-3 |
| Popis: | The influence of a macrolide antibiotic, roxithromycin (RXM), on Th1 and Th2 cytokine productions from human peripheral blood T cells was examined under stimulation with co-stimulatory molecules. Peripheral blood T cells prepared from both healthy and allergic rhinitis donors were cultured in the presence of RXM on anti-CD3 mAb and anti-CD26 mAb-coated wells, anti-CD3 mAb and anti-CD28 mAb-coated wells, and anti-CD3 and PMA. T-cell proliferation, along with the concentration of interleukin (IL)-2, interferon (IFN)-γ, IL-4 and IL-5 were measured. RXM did not affect T-cell proliferation induced by several ways of co-stimulatory activation as assessed by 3H-thymidine incorporation into DNA. RXM also had no effect on IL-2 and IFN-γ secretion by T cells prepared from both healthy and allergic rhinitis donors. On the other hand, RXM markedly inhibited both IL-4 and IL-5 secretions under each of the co-stimulatory conditions in a dose-dependent manner. These results indicate that RXM inhibits specifically Th2 cytokine secretion from T cells induced by co-stimulatory molecule stimulations. This inhibitory action of RXM may be partially responsible for attenuating effect of the agent on the inflammatory diseases. |
| Databáze: | OpenAIRE |
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