Simultaneous sensing of cysteine/homocysteine and glutathione with a fluorescent probe based on a single atom replacement strategy
| Autor: | Haitao Li, Peng Yin, Ting Yu, Jingfeng Yao, Guoxing Yin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Homocysteine 010405 organic chemistry General Chemical Engineering General Engineering Glutathione 010402 general chemistry 01 natural sciences Fluorescence Small molecule Green emission 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Atom Biophysics Cysteine |
| Zdroj: | Analytical Methods. 13:1358-1363 |
| ISSN: | 1759-9679 1759-9660 |
| Popis: | In recent years, there have been many reports of fluorescent probes for multi-channel detection of Cys, Hcy and GSH. Particularly, reports of fluorescent probes using NBD (7-nitro-1,2,3-benzoxadiazole) or SNBD (7-nitro-1,2,3-benzothiadiazole) moieties as fluorophores are particularly common. Unfortunately, their 4-sulfhydryl derivatives exhibited negligible fluorescence, which makes them incapable of detecting GSH directly. Herein, by performing single selenium-for-oxygen atom replacement within 4-chloro-substituted NBD (NBD-Cl), we developed a small molecule fluorescent probe based on a single atom replacement strategy, which enables the probe to be used for simultaneously distinguishing Cys/Hcy and GSH, along with fluorescence imaging of Cys/Hcy and GSH in live cells from red and green emission channels, respectively. |
| Databáze: | OpenAIRE |
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