Both CD34+38+ and CD34+38− Cells Home Specifically to the Bone Marrow of NOD/LtSZ scid/scid Mice but Show Different Kinetics in Expansion
| Autor: | M De Smedt, Bart Vandekerckhove, Tessa Kerre, Fritz Offner, J De Bosscher, Jean Plum, G De Smet |
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| Rok vydání: | 2001 |
| Předmět: |
Annexins
Cell Survival medicine.medical_treatment Immunology CD34 Succinimides Antigens CD34 Apoptosis Bone Marrow Cells Spleen Mice SCID Hematopoietic stem cell transplantation Biology Mice NAD+ Nucleosidase Antigen Antigens CD Cell Movement Mice Inbred NOD medicine Animals Humans Immunology and Allergy ADP-ribosyl Cyclase Cells Cultured Fluorescent Dyes Membrane Glycoproteins Hematopoietic Stem Cell Transplantation Fetal Blood Fluoresceins Hematopoietic Stem Cells ADP-ribosyl Cyclase 1 Antigens Differentiation Molecular biology Kinetics Haematopoiesis medicine.anatomical_structure Bone marrow Stem cell Cell Division Homing (hematopoietic) |
| Zdroj: | The Journal of Immunology. 167:3692-3698 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.167.7.3692 |
| Popis: | Human hemopoietic stem cells (HSC) have been shown to engraft, differentiate, and proliferate in the hemopoietic tissues of sublethally irradiated NOD/LtSZ scid/scid (NOD/SCID) mice. We used this model to study homing, survival, and expansion of human HSC populations from different sources or phenotype. We observed that CD34+ cells homed specifically to bone marrow (BM) and spleen, but by 3 days after injection, survived only in the BM. These BM-homed CD34+ cells proliferated intensively and gave rise to a 12-fold, 5.5-fold, and 4-fold expansion in 3 days for umbilical cord blood, adult mobilized peripheral blood, and adult BM-derived cells, respectively. By injection of purified subpopulations, it was demonstrated that both CD34+38+ and CD34+38− umbilical cord blood HSC homed to the BM and expanded. Importantly, kinetics of expansion were different: CD34+38+ cells started to increase in cell number from day 3 onwards, and by 4 wk after injection, virtually all CD34+ cells had disappeared. In contrast, CD34+38− cells remained quiescent during the first week and started to expand intensively from the third week on. In this paper, we have shown that homing, survival, and expansion of stem cells are three independent phenomena important in the early phase of BM engraftment and that kinetics of engraftment differ between CD34+38+ and CD34+38− cells. |
| Databáze: | OpenAIRE |
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