Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy
| Autor: | Youngjik Choi, Simon J. Attwood, Zoya Leonenko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
force volume
Vesicle fusion Nanotechnology force spectroscopy 02 engineering and technology 010402 general chemistry 01 natural sciences Article Catalysis lcsh:Chemistry Inorganic Chemistry Phase (matter) DOPC DPPC AFM supported lipid bilayer vesicle fusion breakthrough forces Physical and Theoretical Chemistry Lipid bilayer lcsh:QH301-705.5 Molecular Biology Spectroscopy Chemistry Bilayer Organic Chemistry Force spectroscopy Biological membrane General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences Computer Science Applications Membrane lcsh:Biology (General) lcsh:QD1-999 Chemical physics lipids (amino acids peptides and proteins) Mica 0210 nano-technology |
| Zdroj: | International Journal of Molecular Sciences; Volume 14; Issue 2; Pages: 3514-3539 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 14, Iss 2, Pp 3514-3539 (2013) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms14023514 |
| Popis: | Cell membranes are typically very complex, consisting of a multitude of different lipids and proteins. Supported lipid bilayers are widely used as model systems to study biological membranes. Atomic force microscopy and force spectroscopy techniques are nanoscale methods that are successfully used to study supported lipid bilayers. These methods, especially force spectroscopy, require the reliable preparation of supported lipid bilayers with extended coverage. The unreliability and a lack of a complete understanding of the vesicle fusion process though have held back progress in this promising field. We document here robust protocols for the formation of fluid phase DOPC and gel phase DPPC bilayers on mica. Insights into the most crucial experimental parameters and a comparison between DOPC and DPPC preparation are presented. Finally, we demonstrate force spectroscopy measurements on DOPC surfaces and measure rupture forces and bilayer depths that agree well with X-ray diffraction data. We also believe our approach to decomposing the force-distance curves into depth sub-components provides a more reliable method for characterising the depth of fluid phase lipid bilayers, particularly in comparison with typical image analysis approaches. |
| Databáze: | OpenAIRE |
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