Biological activity of bovine placental lactogen in 3T3-F442A preadipocytes is mediated through a somatogenic receptor
| Autor: | Dorit Vashdi, Edna Sakal, Gerard Elberg, Arieh Gertler |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Ovine prolactin
medicine.medical_specialty Biophysics Biology Biochemistry Mice Radioligand Assay Structural Biology Competitive binding Internal medicine Genetics medicine Tumor Cells Cultured Animals Placental lactogen Receptor Molecular Biology 3t3 f442a Human growth hormone Biological activity Affinity Labels Cell Differentiation Affinity labelling Cell Biology 3T3 Cells Receptors Somatotropin 3T3-F442A preadipocyte In vitro Recombinant Proteins Prolactin Endocrinology Adipose Tissue Growth Hormone Somatogenic receptor Cattle Mitogens Cell Division |
| Zdroj: | FEBS Letters. (2):101-104 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(92)80873-F |
| Popis: | Bovine placental lactogen (bPL) exhibited antimitogenic differentiation-promoting, biological activity in 3T3-F442A preadipocytes, Competitive binding studies and affinity labelling revealed bPL activity to be mediated through a somatogenic type of receptor that recognizes human growth hormone (hGH) and bovine GH, but not ovine prolactin or human PL. The bioactivity of bPL was sixfold lower than that of hGH despite that bPL is binding to the somatogenic receptors with fivefold higher affinity. This discrepancy may result from the relatively low ability of bPL to induce post-receptoral effects such as receptor dimerization. |
| Databáze: | OpenAIRE |
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