Polyclonal and monoclonal thyroid nodules coexist within human multinodular goiters
| Autor: | Edna Teruko Kimura, M Oestreicher, Peter Kopp, Simon Aeschimann, Martin F. Fey, Andreas Tobler, Hugo Studer |
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| Rok vydání: | 1994 |
| Předmět: |
Thyroid nodules
Adult Genetic Markers Male endocrine system Pathology medicine.medical_specialty Goiter endocrine system diseases Somatic cell Endocrinology Diabetes and Metabolism Clinical Biochemistry Population Biology Biochemistry Deoxyribonuclease HpaII Pathogenesis Endocrinology Internal medicine Dosage Compensation Genetic medicine Humans Progenitor cell education Deoxyribonucleases Type II Site-Specific Aged education.field_of_study Stem Cells Biochemistry (medical) Middle Aged medicine.disease Clone Cells Blotting Southern Polyclonal antibodies Monoclonal biology.protein Female DNA Probes Polymorphism Restriction Fragment Length Goiter Nodular |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 79(1) |
| ISSN: | 0021-972X |
| Popis: | Although somatic mutations have been identified in a subset of thyroid nodules, the pathogenesis of nodules in multinodular goiters remains unclear. Clonal analysis indicates whether a nodule arises from the polyclonal proliferation of a group of cells or forms a clone from a genetically altered cell. Individual thyroid nodules have been shown to be of polyclonal or monoclonal origin. In this study we examined the clonality of several different nodules in patients with multinodular goiters. Clonality was established using the X-chromosomal probe M27 beta, which detects a multiallelic polymorphism at the locus DXS255 in 90% of females. Twenty-five nodules from 9 multinodular goiters were analyzed; 9 nodules were polyclonal, and 16 were monoclonal. Three goiters contained only polyclonal nodules, whereas 3 contained only monoclonal nodules. Polyclonal and monoclonal nodules coexisted in 3 goiters. In 2 goiters, the monoclonal nodules were shown to derive from different progenitor cells. We conclude that polyclonal and monoclonal nodules may coexist in multinodular goiters and that monoclonal nodules can originate from different cells. The coexistence of polyclonal and monoclonal nodules suggests that different pathogenic mechanisms occur simultaneously or that monoclonal nodules emerge secondarily from a polyclonal population due to a growth advantage from a genetically altered cell. |
| Databáze: | OpenAIRE |
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