Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples
| Autor: | Gabriele Favero, Elisabetta Martini, Giovanni Merola, Luigi Campanella, Mauro Tomassetti, Gabriella Sanzò, Franco Mazzei |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
electrical and electronic engineering ampicillin flow surface plasmon resonance (SPR) immunosensor competitive amperometric immunosensor analytical comparison education 02 engineering and technology Biosensing Techniques lcsh:Chemical technology 01 natural sciences Direct flow Article atomic and molecular physics and optics analytical chemistry biochemistry lcsh:TP1-1185 Surface plasmon resonance Instrumentation Detection limit Immunoassay Chromatography business.industry Natural water 010401 analytical chemistry Surface Plasmon Resonance 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Amperometry 0104 chemical sciences Anti-Bacterial Agents Linear range Optoelectronics 0210 nano-technology business |
| Zdroj: | Sensors; Volume 17; Issue 4; Pages: 819 Sensors, Vol 17, Iss 4, p 819 (2017) Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s17040819 |
| Popis: | In this research, we developed a direct-flow surface plasmon resonance (SPR) immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection (LOD) value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor. |
| Databáze: | OpenAIRE |
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