Distinct cis elements in the 3' UTR of the C. elegans cebp-1 mRNA mediate its regulation in neuronal development
| Autor: | Yishi Jin, Kyung Won Kim, Zilu Wu, Panid Sharifnia |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Five prime untranslated region Messenger Regulatory Sequences Nucleic Acid Medical and Health Sciences mRNA secondary structure Gene expression Translational regulation Developmental Transgenes 3' Untranslated Regions Caenorhabditis elegans Sequence Deletion Genetics Neurons biology Gene Expression Regulation Developmental Biological Sciences Mechanosensory neurons Ubiquitin ligase Stem-loop Phenotype Neurological rpm-1 1.1 Normal biological development and functioning Article 03 medical and health sciences Underpinning research Animals Regeneration RNA Messenger mRNA stability Caenorhabditis elegans Proteins Axon regeneration Molecular Biology Messenger RNA Base Sequence Nucleic Acid Three prime untranslated region Neurosciences Cell Biology biology.organism_classification Axons 030104 developmental biology Gene Expression Regulation Translation regulation biology.protein CCAAT-Enhancer-Binding Proteins RNA Nucleic Acid Conformation Generic health relevance CRISPR-Cas Systems Regulatory Sequences Developmental Biology |
| Zdroj: | Developmental biology, vol 429, iss 1 |
| Popis: | The 3′ untranslated regions (3′ UTRs) of mRNAs mediate post-transcriptional regulation of genes in many biological processes. Cis elements in 3′ UTRs can interact with RNA-binding factors in sequence-specific or structure-dependent manners, enabling regulation of mRNA stability, translation, and localization. Caenorhabditis elegans CEBP-1 is a conserved transcription factor of the C/EBP family, and functions in diverse contexts, from neuronal development and axon regeneration to organismal growth. Previous studies revealed that the levels of cebp-1 mRNA in neurons depend on its 3′ UTR and are also negatively regulated by the E3 ubiquitin ligase RPM-1. Here, by systematically dissecting cebp-1 's 3′ UTR, we test the roles of specific cis elements in cebp-1 expression and function in neurons. We present evidence for a putative stem-loop in the cebp-1 3′ UTR that contributes to basal expression levels of mRNA and to negative regulation by rpm-1 . Mutant animals lacking the endogenous cebp-1 3′ UTR showed a noticeable increased expression of cebp-1 mRNA and enhanced the neuronal developmental phenotypes of rpm-1 mutants. Our data reveal multiple cis elements within cebp-1 's 3′ UTR that help to optimize CEBP-1 expression levels in neuronal development. |
| Databáze: | OpenAIRE |
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