EGR1 supports the osteogenic differentiation of dental stem cells
| Autor: | Oliver Felthaus, Martin Gosau, Christian Morsczeck, T. Press, Sandra Viale-Bouroncle |
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| Rok vydání: | 2014 |
| Předmět: |
endocrine system
Blotting Western Bone Morphogenetic Protein 2 Biology Transfection Bone morphogenetic protein 2 Osteogenesis Humans General Dentistry Transcription factor Early Growth Response Protein 1 Homeodomain Proteins Regulation of gene expression Dental follicle Staining and Labeling Reverse Transcriptase Polymerase Chain Reaction Stem Cells DLX3 Cell Differentiation Dental Sac Alkaline Phosphatase Flow Cytometry Molecular biology Bone morphogenetic protein 6 Alkaline phosphatase Molar Third Transcription Factors |
| Zdroj: | International Endodontic Journal. 48:185-192 |
| ISSN: | 0143-2885 |
| Popis: | Aim To evaluate whether and how the transcription factor early growth response gene 1 (EGR1) affects the osteogenic differentiation of dental stem cells. Methodology Dental stem cells from apical papilla (SCAPs) and from the dental follicle (DFCs) were transfected with EGR1-specific siRNA or EGR-1 expression plasmid. Gene regulation was verified at protein level by Western blotting. The expression of the transcription factors distal-less homeobox 3 (DLX3), alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP2), which are all regulators and markers of the osteogenic differentiation in dental stem cells, was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). To investigate mineralization, SCAP long-term cultures were stained with alizarin red after EGR1 over-expression. Results EGR1 was induced in SCAPs during osteogenic differentiation. DLX3 and bone morphogenetic protein 2 (BMP2) were up-regulated after EGR1 over-expression and down-regulated after EGR1 depletion. The expression of ALP was also down-regulated after EGR1 depletion. The over-expression of EGR1 in SCAPs promoted mineralization after osteogenic differentiation. Conclusions EGR1 supported the osteogenic differentiation of dental stem cells by potentially regulating the expression of DLX3 and BMP2. |
| Databáze: | OpenAIRE |
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