A novel transgenic mice model for venous malformation
| Autor: | Xin Quan Jiang, Zhao Liang Fei, Jian Fei, Jia Wei Zheng, Zhugang Wang, Yan An Wang, Zhiyuan Zhang |
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| Rok vydání: | 2008 |
| Předmět: |
Genetically modified mouse
Pathology medicine.medical_specialty Vascular Malformations Ratón Antigens Polyomavirus Transforming Transgene Genetic Vectors Mice Transgenic Biology Chromosomes Mice In vivo Chlorocebus aethiops Genetics medicine Animals Transgenes Antigen Gene Microinjection Models Genetic Murine polyomavirus medicine.disease Virology Mice Inbred C57BL Disease Models Animal Phenotype Genetic Techniques COS Cells Animal Science and Zoology Venous malformation Agronomy and Crop Science Plasmids Biotechnology |
| Zdroj: | Transgenic Research. 18:193-201 |
| ISSN: | 1573-9368 0962-8819 |
| DOI: | 10.1007/s11248-008-9224-1 |
| Popis: | Vascular anomalies are most commonly seen in the head and neck region, and there is no animal model available of this disease until now. The purpose of this study was to construct a conditional murine polyomavirus middle T antigen gene (PyMT) transgenic mice model, in order to provide a basis for the treatment of vascular anomalies in vivo, as well as the study of PyMT's molecular function. A new conditional transgenic vector based on Tet-On system was constructed successfully. After the experiment in vitro, pronuclear microinjection method was used to introduce the purified transgene into the chromosomes of fertilized mice eggs, and five transgenic positive mice were obtained. The transgenic positive animals went down to future generation by hybridization. After induction of PyMT's expression in the F1 generation, three transgenic mice developed venous malformation which was confirmed histopathologically. The mice model generated could be used as a tool to study venous malformation, as well as the function of PyMT gene. |
| Databáze: | OpenAIRE |
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