Adjuvant effect of mesoporous silica SBA-15 on anti-diphtheria and anti-tetanus humoral immune response
| Autor: | Aryene Góes Trezena, Pedro Leonidas Oseliero Filho, Luís Carlos Cides da Silva, Cristiano Luis Pinto Oliveira, José Luiz de Souza Lopes, Nayara da Silva Antonio, Viktor Fonseca Barbosa Dettmann, Milena Apetito Akamatsu, Tereza da Silva Martins, Orlando Garcia Ribeiro, Márcia Carvalho de Abreu Fantini, Osvaldo Augusto Sant'Anna, Milene Tino-De-Franco |
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| Rok vydání: | 2022 |
| Předmět: |
Pharmacology
Tetanus General Immunology and Microbiology FLUOROMETRIA Immunization Secondary Bioengineering Diphtheria General Medicine Silicon Dioxide Applied Microbiology and Biotechnology Antibodies Bacterial Immunity Humoral Mice X-Ray Diffraction Adjuvants Immunologic Scattering Small Angle Tetanus Toxoid Animals Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Routine immunization against diphtheria and tetanus has drastically reduced the incidence of these diseases worldwide. Anti-diphtheria/tetanus vaccine has in general aluminum salt as adjuvant in its formulation that can produce several adverse effects. There is a growing interest in developing new adjuvants. In this study, we evaluated the efficiency of SBA-15 as an adjuvant in subcutaneous immunization in mice with diphtheria (dANA) and tetanus (tANA) anatoxins as well as with the mixture of them (dtANA). The tANA molecules and their encapsulation in SBA-15 were characterized using Small-Angle X-ray Scattering (SAXS), Dynamical Light Scattering (DLS), Nitrogen Adsorption Isotherm (NAI), Conventional Circular Dichroism (CD)/Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy, and Tryptophan Fluorescence Spectroscopy (FS). The primary and secondary antibody response elicited by subcutaneous immunization of High (H |
| Databáze: | OpenAIRE |
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