Neural agrin controls acetylcholine receptor stability in skeletal muscle fibers
| Autor: | Terje Lømo, Guido Francesco Fumagalli, Iren Sefland, Inger Rabben, Gabriela Bezakova |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty animal structures DNA Complementary Electrical muscle stimulation medicine.medical_treatment Recombinant Fusion Proteins Muscle Fibers Skeletal Neuromuscular Junction Muscle Proteins Stimulation Nerve Tissue Proteins Biology Transfection Neuromuscular junction Internal medicine medicine Animals Receptors Cholinergic Agrin Rats Wistar Receptor Muscle Skeletal Acetylcholine receptor Denervation Multidisciplinary Biological Sciences musculoskeletal system Bungarotoxins Electric Stimulation Cell biology Rats Endocrinology medicine.anatomical_structure nervous system Half-Life |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 98(17) |
| ISSN: | 0027-8424 |
| Popis: | At mammalian neuromuscular junctions (NMJs), innervation induces and maintains the metabolic stability of acetylcholine receptors (AChRs). To explore whether neural agrin may cause similar receptor stabilization, we injected neural agrin cDNA of increasing transfection efficiencies into denervated adult rat soleus (SOL) muscles. As the efficiency increased, the amount of recombinant neural agrin expressed in the muscles also increased. This agrin aggregated AChRs on muscle fibers, whose half-life increased in a dose-dependent way from 1 to 10 days. Electrical muscle stimulation enhanced the stability of AChRs with short half-lives. Therefore, neural agrin can stabilize aggregated AChRs in a concentration- and activity-dependent way. However, there was no effect of stimulation on AChRs with a long half-life (10 days). Thus, at sufficiently high concentrations, neural agrin alone can stabilize AChRs to levels characteristic of innervated NMJs. |
| Databáze: | OpenAIRE |
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