Biotechnological Potential of Araucaria angustifolia Pine Nuts Extract and the Cysteine Protease Inhibitor AaCI-2S
| Autor: | Thiago Henrique Napoleão, Rosemeire A. Silva-Lucca, Patrícia Maria Guedes Paiva, Rodrigo da Silva Ferreira, Maria Luiza Vilela Oliva, Roberto C. Sallai, Bruno Ramos Salu, Flávio Lopes Alves, Misako U. Sampaio |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bromelain Proteases plant extracts medicine.medical_treatment cysteine protease inhibitor termites tumor cells Araucaria angustifolia Plant Science urban pest Cathepsin L functional food 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Botany medicine Ecology Evolution Behavior and Systematics Protease bioactive compounds Ecology biology Chemistry insecticide Cysteine protease Protease inhibitor (biology) lcsh:QK1-989 pine nuts Papain 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis biology.protein medicine.drug Cysteine |
| Zdroj: | Plants, Vol 9, Iss 1676, p 1676 (2020) Plants Volume 9 Issue 12 |
| ISSN: | 2223-7747 |
| Popis: | Protease inhibitors are involved in the regulation of endogenous cysteine proteases during seed development and play a defensive role because of their ability to inhibit exogenous proteases such as those present in the digestive tracts of insects. Araucaria angustifolia seeds, which can be used in human and animal feed, were investigated for their potential for the development of agricultural biotechnology and in the field of human health. In the pine nuts extract, which blocked the activities of cysteine proteases, it was detected potent insecticidal activity against termites (Nasutitermes corniger) belonging to the most abundant termite genus in tropical regions. The cysteine inhibitor (AaCI-2S) was purified by ion-exchange, size exclusion, and reversed-phase chromatography. Its functional and structural stability was confirmed by spectroscopic and circular dichroism studies, and by detection of inhibitory activity at different temperatures and pH values. Besides having activity on cysteine proteases from C. maculatus digestive tract, AaCI-2S inhibited papain, bromelain, ficin, and cathepsin L and impaired cell proliferation in gastric and prostate cancer cell lines. These properties qualify A. angustifolia seeds as a protein source with value properties of natural insecticide and to contain a protease inhibitor with the potential to be a bioactive molecule on different cancer cells. |
| Databáze: | OpenAIRE |
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