Expression of FOXO6 is Associated With Oxidative Stress Level and Predicts the Prognosis in Hepatocellular Cancer
| Autor: | Fu-Chun Yang, Shusen Zheng, Xiaofeng Xu, Yao-Min Chen, Hai-Yong Chen, Jian Wu, Zhen Lv |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine HBsAg Pathology Apoptosis medicine.disease_cause 0302 clinical medicine Cyclin D1 chemistry.chemical_classification biology Glutathione peroxidase Liver Neoplasms Forkhead Transcription Factors Clinical Trial/Experimental Study General Medicine Middle Aged Catalase Prognosis 030220 oncology & carcinogenesis Immunohistochemistry Female alpha-Fetoproteins Liver cancer Research Article Adult medicine.medical_specialty Andrology Superoxide dismutase 03 medical and health sciences medicine Humans Aged Cell Proliferation Neoplasm Staging Glutathione Peroxidase Reactive oxygen species Superoxide Dismutase business.industry medicine.disease digestive system diseases Oxidative Stress 030104 developmental biology chemistry Heme Oxygenase (Decyclizing) biology.protein Reactive Oxygen Species business Oxidative stress |
| Zdroj: | Medicine |
| ISSN: | 0025-7974 |
| Popis: | The aim of this study was to explore the association of Forkhead box O6 (FOXO6) expression with oxidative stress level and prognosis of hepatocellular cancer (HCC). The case group included tissues of HCC from 128 patients who were hospitalized in Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery of First Affiliated Hospital, School of Medicine, Zhejiang University. The control group included normal liver tissues from 74 patients. RT-PCR and Western blot were used to test expressions of FOXO6, heme oxygenase (HO)-1, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). Dihydroethidium (DHE) was dyed to observe reactive oxygen species (ROS) level. Immunohistochemistry was used to test FOXO6 expression. FOXO6 was silenced in HepG2 cells to detect cell proliferation and apoptosis. The expressions of ROS, HO-1, GPx, SOD, CAT, p27, and cyclin D1 were also detected to further explore the possible mechanism. The expressions of FOXO6, HO-1, GPx, SOD, and CAT in HCC tissue was significantly higher than those in normal and adjacent HCC tissues (P |
| Databáze: | OpenAIRE |
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